Project description:Here, we show that the Kdm5c/Smcx member of the Jarid1 family of H3K4 demethylases is recruited to both enhancer and core promoter elements in ES and neuronal progenitor cells (NPC). Knockdown of Kdm5c deregulates transcription via a local increase in H3K4me3. While at core promoters the function of Kdm5c is to restrict transcription, loss of Kdm5c impairs enhancer function. Remarkably, an impaired enhancer function activates promoter activity from Kdm5c-bound intergenic regions. Our results demonstrate that the Kdm5c demethylase plays a crucial role in the functional identity and discrimination of enhancers and core promoters. We speculate that this is related to recruitment of H3K4me3 binders like the TFIID and NURF complexes6-8. Providing functional identity to genomic regions through balancing enzymes that deposit and remove histone modifications may prove to be a general epigenetic mechanism for the functional indexing of eukaryotic genomes. Examination of the KDM5C binding sites in mouse embryonic stem cells and in neuronal progenitor cells. Effect of KDM5C knock down on H3K4me3 and H3K4me1 levels and gene expression.

2013-04-01 | E-GEOD-34975 | biostudies-arrayexpress

Darolutamide antagonizes androgen signaling by blocking enhancer and super-enhancer activation

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-10 | GSE148400 | GEO

The role of Kdm5c

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-09-07 | GSE148001 | GEO

Role of histone demethylase Kdm6a in pancreatic cancer

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-04-01 | GSE98067 | GEO

Aggression, cognitive and neurodevelopmental abnormalities associated with disruption of the intellectual disability and histone demethylase gene Kdm5c

Project description:This SuperSeries is composed of the SubSeries listed below.