Transcriptome profiling of the testis developing and spermatogenesis of Mongolian horse using RNA-Seq
Ontology highlight
ABSTRACT:
ORGANISM(S): Equus caballus
PROVIDER: GSE101697 | GEO | 2017/07/21
SECONDARY ACCESSION(S): PRJNA395221
REPOSITORIES: GEO
Similar Datasets
Project description:PIWI-interacting RNAs (piRNAs), have been identifed in mammalian testes, and were essential for development of testis and spermatogenesis.
2017-07-07 | GSE100852 | GEO
Project description: Not available
| S-EPMC7187496 | biostudies-literature
Project description:Our experimental design includes samples at different time points during the first wave of spermatogenesis. Each time point corresponds to specific cell content in the testis. First time point is post natal day (PND) 7, when only somatic cells and spermatogonia are present in the testis. Thereafter additional cell types are present in selected samples in chronological order: PND 14 contains early spermatocytes, PND 17 late spermatocytes, PND 21 round spermatids and finally PND 28 elongating spermatids. Our results highlight differentially expressed genes and gene isoforms, which are important for correct sperm development and male fertility. In addition, functional analysis suggests a highly controlled gene expression pattern during the first wave of spermatogenesis. Transcriptome sequencing of the mouse testes at PND 7, 14, 17, 21 and 28 for analysis of differences in gene expression pattern during the first wave of spermatogenesis. In total 10 samples were analysed, replicates for each time point.
2013-04-29 | E-GEOD-39970 | biostudies-arrayexpress
Project description:The Mongolian horse has the characteristics of cold resistance, rough feeding resistance, good endurance, strong disease resistance and strong gregariousness. The normal production of sperm in male mammals is the basis of reproduction, and spermatogenesis includes a mitosis, meiosis twice and metamorphosis of sperm cells. The MEI1 gene is involved in the meiosis cycle and is required for normal meiosis chromosome association. Previous studies have shown that alternative splicing of MEI1 gene can promote spermatogenesis in Mongolian horses. In this study, the expression of MEI1 gene was determined by immunofluorescence in the third generation Mongolian horse testicular sertoli cells. Two overexpressed lentiviral vectors with MXE and SE events of MEI1 gene were constructed and successfully infected into sertoli cells. CCK-8 was used to detect the proliferation and activity of infected cells, transcripomics and metabolomics were used to screen and annotate differential genes and differential metabolites in the two sertoli cells with MXE and SE events. After 72 h of virus infection, 193 differentially expressed genes and 11360 differentially expressed metabolites were screened, 109 up-regulated genes including MEI1 and 84 down-regulated genes, 7494 up-regulated metabolites and 3866 down-regulated metabolites. Differential genes and differential metabolites were mainly enriched in several pathways related to spermatogenesis. Differential genes such as IL31RA, ATP2B3, CASQ2 were highly expressed in SE events, IL11, PRLR, CCR7 were highly expressed in MXE events. Metabolites such as folic acid and spermine were highly expressed in SE events, citric acid and glutathione were highly expressed in MXE events. This suggested that both MXE and SE events of MEI1 gene can promote the activity of spermatogenesis signaling pathway, and the gene influences spermatogenesis by regulating the expression of metabolites. In summary, MXE and SE events of MEI1 gene can promote the expression of spermatogen-related genes and metabolites, and genes affect spermatogenesis by regulating the expression of metabolites. The results of this study laid a theoretical foundation for exploring the regulatory mechanism of different alternative splicing events on spermatogenesis in Mongolian horse.
2024-02-02 | GSE254391 | GEO
Project description:Cultivation of neonatal mouse testis tissue can induce spermatogenesis and produce fertile sperms. However, this in vitro spermatogenesis by the current organ culture method comes short in fully mimicking the in vivo counterpart, partly due to a lack of knowledge about the underlying molecular phenotypes. In this study, we investigated transcriptome of cultured testis tissues using microarray method. Principle component analysis of the transcriptome data revealed delay and/or arrest of spermatogenesis and immediate radical immune reactions in the cultured testis tissues. The delay/arrest of spermatogenesis occurred before and during early meiotic phase, resulting in inefficient progression of meiosis. The immune reaction, on the other hand, was drastic and overwhelming, in which TLR4-NF-kB signaling was speculated to be involved. Notably, treatment with TAK242, an inhibitor of TLR4-NF-kB signaling pathway, ameliorated the macrophage activation which otherwise would exacerbate the inflammation. The present study revealed for the first time the immense immune reaction in the cultured tissue by transcriptomic analysis, which could be a cause of the inefficiency of in vitro spermatogenesis.
2020-06-30 | GSE147982 | GEO
Project description:The goat of this project is to explore lncRNA5251 regulation spermatogenesis. We tried to search the mechanism oflncRNA5251 regulation spermatogenesis. RNA-seq of F1 mouse testis samples from different groups: 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knocdown letivirus.
2021-12-01 | GSE142725 | GEO
Project description:The goat of this project is to explore lncRNA5251 regulation spermatogenesis. We tried to search the mechanism oflncRNA5251 regulation spermatogenesis. RNA-seq of F0 mouse testis samples from different groups: 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA5251 overexpression, 3NC, lncRNA5251 knocdown letivirus.
2021-12-01 | GSE142724 | GEO
Project description:The goat of this project is to explore lncRNA8276 regulation spermatogenesis. We tried to search the mechanism oflncRNA8276 regulation spermatogenesis. RNA-seq of F1 mouse testis samples from different groups: 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knocdown letivirus.
2021-12-01 | GSE142795 | GEO
Project description:The goat of this project is to explore lncRNA8276 regulation spermatogenesis. We tried to search the mechanism oflncRNA8276 regulation spermatogenesis. RNA-seq of F0 mouse testis samples from different groups: 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knockdown. The testes of mouse were transfected with virus with 5NC, lncRNA8276 overexpression, 3NC, lncRNA8276 knocdown letivirus.
2021-12-01 | GSE142793 | GEO
Project description:Studying spermatogenesis is the key to understanding the development mechanism of the yak reproductive system. Although N6-methyladenosine (m6A) RNA modification has been reported to regulate spermatogenesis and reproductive function in mammals, the molecular mechanism of m6A in yak testis development and spermatogenesis remains largely unknown. Therefore, we collected testicular tissue from junile and adult yaks, and found that the m6A level significantly increased after sexual maturity in yak. In MeRIP-seq, 1,702 hypermethylated peaks and 724 hypomethylated peaks were identified. The hypermethylated differentially methylated RNAs (DMRs) (CIB2, AK1, FOXJ2, PKDREJ, SLC9A3, and TOPAZ1) mainly regulated spermatogenesis, while the hypomethylated DMRs (BCL6, USP25, CD96, EPHA2, and TAF12) mainly participated in immune response. Functional enrichment analysis showed that DMRs were significantly enriched in the adherens junction, gap junction, and Wnt, PI3K, and mTOR signaling pathways, regulating cell development, spermatogenesis, and testicular endocrine function. The functional analysis of differentially expressed genes showed that they were involved in the biological processes of mitosis, meiosis, and flagellated sperm motility during sexual maturity of yak testis. We also screened the key regulatory factors of testis development and spermatogenesis by combined analysis, which included BRCA1, CREBBP, STAT3, and SMAD4. This study has important research value and practical guiding significance for yak genetic improvement, particularly in understanding the molecular mechanism and evolutionary dynamics of the progression of testis developmental stages in yaks.
2023-04-15 | GSE229363 | GEO