Dataset Information


RNA-sequencing of Mbd3-null and control haematopoietic stem cell and lymphoid progenitor cell populations

ABSTRACT: To determine the role of Mbd3/NuRD in lymphopoiesis, gene expression in purified populations of Mbd3-deleted and control lymphoid progenitor cells was analysed using RNA-seq. Overall design: Mbd3-deficient and control lymphoid progenitors were isolated from mouse bone marrow by flow cytometry, including haematopoietic stem cells (HSCs), lymphoid-primed multipotent progenitors (LMPPs), all-lymphoid progenitors (ALPs) and B cell-biased lymphoid progenitors (BLPs). RNA-seq was performed on 100 HSCs or 150 cells from the other populuations, using the previously described smartseq2 protocol for RNA-seq of small numbers of cells (Picelli et al. (2014) Nature protocols 9:171).

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Stephen John Loughran  

PROVIDER: GSE101732 | GEO | 2017-07-22



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Mbd3/NuRD controls lymphoid cell fate and inhibits tumorigenesis by repressing a B cell transcriptional program.

Loughran Stephen J SJ   Comoglio Federico F   Hamey Fiona K FK   Giustacchini Alice A   Errami Youssef Y   Earp Eleanor E   Göttgens Berthold B   Jacobsen Sten Eirik W SEW   Mead Adam J AJ   Hendrich Brian B   Green Anthony R AR  

The Journal of experimental medicine 20170912 10

Differentiation of lineage-committed cells from multipotent progenitors requires the establishment of accessible chromatin at lineage-specific transcriptional enhancers and promoters, which is mediated by pioneer transcription factors that recruit activating chromatin remodeling complexes. Here we show that the Mbd3/nucleosome remodeling and deacetylation (NuRD) chromatin remodeling complex opposes this transcriptional pioneering during B cell programming of multipotent lymphoid progenitors by r  ...[more]

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