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Oncogenes hijack the stress response machinery in T cell acute lymphoblastic leukemia [GRO-Seq]

ABSTRACT: Oncogenes NOTCH1 and MYC directly regulate HSF1 and other critical proteins of the stress-response pathway in T-ALL. This GRO-Seq experiment demonstrates that release from NOTCH1 inhibition results in upregulation of HSF1 and other key HSPs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE101803 | GEO | 2018/03/20

REPOSITORIES: GEO

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Hijacking of stress response machinery by oncogenes in acute leukaemia [RNA-seq]

Project description:Our data demonstrate binding of NOTCH1 on the promoters of HSF1 and HSF1 targets. We performed RNA-seq upon control conditions, inhibition of the NOTCH1 pathway by gSI, HSF1 knockdown and combination of HSF1 knockdown and gSI treatment. Our results show that NOTCH1 regulates the expression of heat shock response genes. Moreover, combination of HSF1 downregulation and gSI treatment further decreased the expression of heat shock response genes compared to single treatments.

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Sivery2016 - A mammalian heat shock response model

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