Transcriptomics,Genomics

Dataset Information

151

Transcriptional changes during naturally-acquired ZIKA Virus infection render dendritic cells highly conducive to viral replication


ABSTRACT: Although dendritic cells (DCs) are among the human cell population best equipped for cell-intrinsic antiviral immune defense, they seem highly susceptible to infection with Zika Virus (ZIKV). Using highly-purified mDC isolated from individuals with naturally-acquired acute infection, we here show that ZIKV induces profound perturbations of transcriptional signatures relative to uninfected patients. Interestingly, we noted a remarkable downregulation of antiviral Interferon-stimulated genes and innate immune sensors, suggesting that ZIKV can actively suppress Interferon-dependent immune responses. In contrast, several host factors known to support ZIKV infection were strongly upregulated during natural ZIKV infection; these transcripts included AXL, the main entry receptor for ZIKV, SOCS3, a negative regulator of ISG expression, and IDO-1, a recognized inducer of regulatory T cell responses. Thus, during in vivo infection, ZIKV can transform the transcriptome of dendritic cells in favor of the virus to render these cells highly conducive to ZIKV infection. Overall design: Examination of transcriptional changes in myeloid dendritic cells between ZIKV-infected patients and healthy donors

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

SUBMITTER: Xu G Yu  

PROVIDER: GSE101878 | GEO | 2017-12-18

SECONDARY ACCESSION(S): PRJNA395770

REPOSITORIES: GEO

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Publications

Transcriptional Changes during Naturally Acquired Zika Virus Infection Render Dendritic Cells Highly Conducive to Viral Replication.

Sun Xiaoming X   Hua Stephane S   Chen Hsiao-Rong HR   Ouyang Zhengyu Z   Einkauf Kevin K   Tse Samantha S   Ard Kevin K   Ciaranello Andrea A   Yawetz Sigal S   Sax Paul P   Rosenberg Eric S ES   Lichterfeld Mathias M   Yu Xu G XG  

Cell reports 20171201 12


Although dendritic cells are among the human cell population best equipped for cell-intrinsic antiviral immune defense, they seem highly susceptible to infection with the Zika virus (ZIKV). Using highly purified myeloid dendritic cells isolated from individuals with naturally acquired acute infection, we here show that ZIKV induces profound perturbations of transcriptional signatures relative to healthy donors. Interestingly, we noted a remarkable downregulation of antiviral interferon-stimulate  ...[more]

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