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Improvement of Lactobacillus plantarum Aerobic Growth by Comprehensive Transcriptome Analysis

ABSTRACT: An aerobic Lactobacillus plantarum culture displayed growth stagnation during early growth. Transcriptome analysis revealed that regain of growth after stagnation correlated with activation of CO2-producing pathways suggesting that limiting CO2-concentration induced stagnation. Analogously providing increased CO2 gas partial pressure during aerobic fermentation prevented the temporal growth stagnation. Keywords: cell type comparison

ORGANISM(S): Lactiplantibacillus plantarum WCFS1

PROVIDER: GSE10194 | GEO | 2008/01/18

SECONDARY ACCESSION(S): PRJNA108307

REPOSITORIES: GEO

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Improvement of Lactobacillus plantarum Aerobic Growth by Comprehensive Transcriptome Analysis

Project description:An aerobic Lactobacillus plantarum culture displayed growth stagnation during early growth. Transcriptome analysis revealed that regain of growth after stagnation correlated with activation of CO2-producing pathways suggesting that limiting CO2-concentration induced stagnation. Analogously providing increased CO2 gas partial pressure during aerobic fermentation prevented the temporal growth stagnation. Keywords: cell type comparison Two aerobic fermenters (biological replicates Land R) were sampled at two time points, before (1) and after (2) growth stagnation.

2010-05-26 | E-GEOD-10194 | biostudies-arrayexpress

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2010-05-16 | E-GEOD-18432 | biostudies-arrayexpress

Lactiplantibacillus plantarum WCFS1

Project description:Improvement of Lactobacillus plantarum Aerobic Growth by Comprehensive Transcriptome Analysis

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Project description:This data set contains samples from Lactobacillus plantarum WCFS1 wild type and its CcpA mutant derivative. Both strains were grown in duplicate (samples L and R) and at four different growth stages samples were taken (early, mid and late log phase, early stationary phase). Samples were compared to each other within one strain and between the two strains. This allows the comparison of the wild type strain to its CcpA derivative in time. Further it allows comparison within one strain during different growth stages.

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Lifestyle of lactobacillus plantarum in the mouse cecum

Project description:Lactobacillus plantarum is a common inhabitant of mammalian gastrointestinal tracts and specific strains belonging to this species are marketed as probiotics intended to confer beneficial health effects. To assist in determining the physiological status and host-microbe interactions of L. plantarum in the digestive tract we assessed changes in the transcriptome of L. plantarum WCFS1 during colonization of the cecum of germ-free mice. According to the transcript profiles L. plantarum WCFS1 was metabolically active and not under severe stress in this intestinal compartment. Carbohydrate metabolism was the most strongly affected functional gene category whereby many genes encoding diverse sugar transport and degradation pathways were induced in mice even compared to L. plantarum grown in a mouse chow-derived laboratory medium. This suggests that the ability of L. plantarum WCFS1 to consume diverse energy sources including plant-associated and host-derived carbohydrates was increased during its residence in the digestive tract. Many of these genes were also induced in L. plantarum colonizing germ-free mice fed a humanized Western-style diet. Similarly a core set of genes encoding cell surface-related properties were differentially expressed in mice. This set includes genes required for the D-alanylation and glycosylation of lipoteichoic acids that were strongly down-regulated in mice. In total L. plantarum exhibits a distinct in vivo transcriptome directed towards adaptation to the mouse intestinal environment. Keywords: cell type comparison

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2015-04-24 | E-GEOD-68188 | biostudies-arrayexpress

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