Dataset Information


H3K79me2 ChIP-seq in cerebellar granular neurons (CGN) and progenitors (CGNP) upon DOT1L inhibition with SGC0946 in vitro

ABSTRACT: DOT1L as methyltransferase of H3K79 is implicated in brian development. Here, we further defined DOT1L function within the granular neurons during cerebellar development using ChIP-seq of H3K79 dimethylation of isolated cerebellar granular neurons and progenitors. Thereby we compared samples treated with a DOT1L inhibitor versus DMSO treated cells. The data sets reveals new important targets of DOT1L, which ensure a correct development of the cerebellum. Overall design: H3K79me2 ChIP-seq of CGNPs,4 h after DOT1L inhibition and CGNs, 44 h after DOT1L inhibition; compared to DMSO control each. Cells derived from murine cerebellum of P7 NMRI WT mice

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Patrick Piero Bovio 

PROVIDER: GSE101947 | GEO | 2018-10-15


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Differential Methylation of H3K79 Reveals DOT1L Target Genes and Function in the Cerebellum In Vivo.

Bovio Patrick Piero PP   Franz Henriette H   Heidrich Stefanie S   Rauleac Tudor T   Kilpert Fabian F   Manke Thomas T   Vogel Tanja T  

Molecular neurobiology 20181010 6

The disruptor of telomeric silencing 1-like (DOT1L) mediates methylation of histone H3 at position lysine 79 (H3K79). Conditional knockout of Dot1l in mouse cerebellar granule cells (Dot1l-cKOAtoh1) led to a smaller external granular layer with fewer precursors of granule neurons. Dot1l-cKOAtoh1 mice had impaired proliferation and differentiation of granular progenitors, which resulted in a smaller cerebellum. Mutant mice showed mild ataxia in motor behavior tests. In contrast, Purkinje cell-spe  ...[more]

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