Dataset Information


Transcriptomic analysis of in vitro-generated human monocyte-derived cells

ABSTRACT: We analyzed the transcriptomes of human dendritic cells and macrophages derived from monocytes using MCSF + IL-4 + TNFa, or IL-34 + IL-4 + TNFa, or dendritic cells derived from monocytes using GMCSF + IL-4. Overall design: We used as precursor cells the monocytes purified from the blood of 6 different donors. The data set contains therefore 6 biological replicates for each population of monocyte-derived cells analyzed.

INSTRUMENT(S): [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]

SUBMITTER: Elodie Segura  

PROVIDER: GSE102046 | GEO | 2017-09-15



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After entering tissues, monocytes differentiate into cells that share functional features with either macrophages or dendritic cells (DCs). How monocyte fate is directed toward monocyte-derived macrophages (mo-Macs) or monocyte-derived DCs (mo-DCs) and which transcription factors control these differentiation pathways remains unknown. Using an in vitro culture model yielding human mo-DCs and mo-Macs closely resembling those found in vivo in ascites, we show that IRF4 and MAFB were critical regul  ...[more]

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