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Ectopic expression of the Brachyury transcription factor upregulates only a subset of notochord expressed genes

ABSTRACT: The key notochord transcription factor Brachyury was ectopically expressed in Ciona embryos under the control of the FoxAa cis-regulatory region (which drives expression in neural, endodermal and mesenchymal lineages in addition to notochord). Misexpression of Brachyury induced 925 genes compared to a control reporter plasmid (Bra>GFP). There was only modest overlap with a set of notochord-enriched genes previously identified by RNAseq of flow-sorted notochord cells, indicating that Brachyury is not a notochord master regulator gene as strictly defined.

ORGANISM(S): Ciona intestinalis

PROVIDER: GSE102142 | GEO | 2017/09/19

SECONDARY ACCESSION(S): PRJNA396771

REPOSITORIES: GEO

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Brachyury controls Ciona notochord fate as part of a feedforward network [III]

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