Transcriptomics,Genomics

Dataset Information

35

Keratinocyte specific Fos-deletion in K5-Sos-F mouse tumor model


ABSTRACT: Expression and function of the oncogenic transcription factor AP-1 (mainly composed of Jun and Fos proteins) is required for neoplastic transformation of mouse and human keratinocytes in vitro and tumor promotion as well as malignant progression in vivo. Here, we describe the identification of novel Fos target genes using global gene expression profiling with samples from a tumor model of mouse skin (K5-SOS-F). We could identify 366 differentially expressed genes comparing expression profiles from tumor samples of control animals with samples derived form mice with a specific deletion of fos in keratinocytes. Keywords: Fos-deletion, Fos-floxed, K5-SOS-F mouse tumor model, skin papilloma, global gene expression, microarray, Fos target in skin carcinogenesis Overall design: In the present Experiment two different genotypes in combination with an established mouse tumor model were compared. An active form of the Sos (son of sevenless) is expressed under a keratin5 promoter leading to a constitutive activation of the Ras-Raf pathway, which results in skin papilloma formation. In the context of this tumor model an epidermis specific homozygote Fos-deletion was compared to homozygous floxed Fos-alleles. Both genotypes (Fos-deleted and Fos-floxed) were treated equally. The specimens were taken from skin papilloma at the mouse tail. A total number of six specimens from six different individuals (three biological replicates per genotype) were taken. Each sample was prepared and hybridized against Universal Mouse Reference RNA (Stratagene) in a dye-swap experiment, resulting in two technical replicates for each specimen, respectively.

INSTRUMENT(S): Operon Mouse 36k v4.0

SUBMITTER: Daniel Haag  

PROVIDER: GSE10218 | GEO | 2008-09-01

SECONDARY ACCESSION(S): PRJNA108277

REPOSITORIES: GEO

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Publications

Podoplanin is a novel fos target gene in skin carcinogenesis.

Durchdewald Moritz M   Guinea-Viniegra Juan J   Haag Daniel D   Riehl Astrid A   Lichter Peter P   Hahn Meinhard M   Wagner Erwin F EF   Angel Peter P   Hess Jochen J  

Cancer research 20080901 17


Expression and function of the oncogenic transcription factor activator protein (AP-1; mainly composed of Jun and Fos proteins) is required for neoplastic transformation of keratinocytes in vitro and tumor promotion as well as malignant progression in vivo. Here, we describe the identification of 372 differentially expressed genes comparing skin tumor samples of K5-SOS-F transgenic mice (Fos(f/f) SOS(+)) with samples derived from animals with a specific deletion of c-Fos in keratinocytes (Fos(De  ...[more]

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