Transcriptomics,Genomics

Dataset Information

156

RNA-Seq transcriptome profiling of soleus from adult and aged mice following disuse muscle atrophy and recovery


ABSTRACT: Rodent hind limb unloading was used as a model for reduced muscle activity and eventual atrophy. After a 10 day period of unloading, mice in this study were “reloaded” for 3 days and regained use of their hind limbs. We report the application of Next-generation sequencing (NGS) technology for high-throughput profiling of mRNA in soleus muscle of adult (6 mo) and aged (22-24 mo) mice. Our goal was to determine the effects of hind limb unloading and reloading on mRNA profiles in soleus muscle and compare between adult and aged mice. We find that there are distinct response in the profile of fatty acid oxidation, TCA cycle, ETC oxidative phosphorylation gene expression patterns in response to unloading and reloading. The repsonses are generally simialr between young and old mice. Overall design: RNA-Seq was performed with total RNA isolated from snap frozen soleus muscle. Male C57BL/6J mice (Adult 6 mo and Old 22-24 mo) were obtained from the NIA's Aged Rodent Colony Charles River Laboratories, Madison, WI) and were subjected to the following experimental conditions: 1) hind limb unloaded for 10 days, 2) hind limb unloaded for 10 days and reloaded for 3 days, or 3) maintained under control conditions for 10 days. Mice were fed ad libitum with a standard chow diet (#2916, Harlan-Teklad, Houston, Tx) and water/hydrogel (ClearH2O, Westbrook, ME, US) and housed at 22C with a 12-h light/dark cycle. All soleus samples were harvested following a 4 hour fast and mice were sacrificed at ~11am by CO2 asphyxiation and cervical dislocation.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Paul M Coen  

PROVIDER: GSE102284 | GEO | 2018-05-15

REPOSITORIES: GEO

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Publications

Impaired Mitochondrial Energetics Characterize Poor Early Recovery of Muscle Mass Following Hind Limb Unloading in Old Mice.

Zhang Xiaolei X   Trevino Michelle B MB   Wang Miao M   Gardell Stephen J SJ   Ayala Julio E JE   Han Xianlin X   Kelly Daniel P DP   Goodpaster Bret H BH   Vega Rick B RB   Coen Paul M PM  

The journals of gerontology. Series A, Biological sciences and medical sciences 20180901 10


The progression of age-related sarcopenia can be accelerated by impaired recovery of muscle mass following periods of disuse due to illness or immobilization. However, the mechanisms underlying poor recovery of aged muscle following disuse remain to be delineated. Recent evidence suggests that mitochondrial energetics play an important role in regulation of muscle mass. Here, we report that 22- to 24-month-old mice with low muscle mass and low glucose clearance rate also display poor early recov  ...[more]

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