Transcriptomics,Genomics

Dataset Information

97

Resistance to pentamidine is mediated by AdeAB, regulated by AdeRS, and influenced by growth conditions in Acinetobacter baumannii ATCC 17978


ABSTRACT: The goal of this study was to assess the role of the AdeRS two component signal transduction system in Acinetobacter baumannii ATCC 17978 and its impact on the virulence potential of this bacterial organism. Specific gene deletion strains targeting the adeRS system were generated and compared to wildtype for a number of phenotypic in vitro assays as well as transcriptomic profiling via RNA-sequencing. Overall design: Examination of ∆adeRS mutant derivative compared to wildtype A. baumannii ATCC 17978 from bacterial cells grown in Mueller-Hinton broth which were grown to 0.6 OD600. Total RNA was isolated in triplicate samples over three biological replicates. RNA-sequencing was undertaken on pooled RNA. The overall aim was to assess the global transcriptional profile of the A. baumannii ATCC 17978 ∆adeRS two component system deletion strain in comparison to the transcriptome of wildtype A. baumannii ATCC 17978.

INSTRUMENT(S): Illumina HiSeq 2000 (Acinetobacter baumannii ATCC 17978)

SUBMITTER: Felise Adams  

PROVIDER: GSE102711 | GEO | 2018-05-13

REPOSITORIES: GEO

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Publications

Resistance to pentamidine is mediated by AdeAB, regulated by AdeRS, and influenced by growth conditions in Acinetobacter baumannii ATCC 17978.

Adams Felise G FG   Stroeher Uwe H UH   Hassan Karl A KA   Marri Shashikanth S   Brown Melissa H MH  

PloS one 20180511 5


In recent years, effective treatment of infections caused by Acinetobacter baumannii has become challenging due to the ability of the bacterium to acquire or up-regulate antimicrobial resistance determinants. Two component signal transduction systems are known to regulate expression of virulence factors including multidrug efflux pumps. Here, we investigated the role of the AdeRS two component signal transduction system in regulating the AdeAB efflux system, determined whether AdeA and/or AdeB c  ...[more]

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