Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA regions bound by Myxococcus xanthus ParB and FrzCD

ABSTRACT: Our ChipSeq analysis show that while FrzCD does not bind DNA specific regions, ParB binds parS consistent with what as been previously shown. The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. FrzCD directly binds to the nucleoid and the FrzCD binding to the DNA leads to the formation of chemosensory complexes. This supra-molecular organization is required for cooperative interactions between clustered receptors, in turn important for the modulation of bacterial social behaviors.

ORGANISM(S): Myxococcus xanthus

PROVIDER: GSE102724 | GEO | 2018/02/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Myxococcus xanthus

Project description:DNA regions bound by Myxococcus xanthus ParB and FrzCD

| PRJNA398503 | ENA

Myxococcus xanthus strain:DK1622

Project description:Myxococcus xanthus

| PRJNA266287 | ENA

Myxococcus xanthus

Project description:Myxococcus xanthus Raw sequence reads

| PRJNA824127 | ENA

Myxococcus xanthus

Project description:Myxococcus xanthus Raw sequence reads

| PRJNA314564 | ENA

Myxococcus xanthus

Project description:Myxococcus xanthus Genome sequencing and assembly

| PRJNA630034 | ENA

c-di-GMP-induced conformational dynamics of the c-di-GMP-binding protein A (CdbA) from Myxococcus xanthus

Project description:The second messenger c-di-GMP has important functions in response to changing environmental and cellular cues in bacteria. Here, we report the identification of CdbA, a DNA binding protein of the ribbon-helix-helix family in Myxococcus xanthus, and show that it binds c-di-GMP in vitro. CdbA is essential for viability and its depletion caused defects in chromosome organization and segregation resulting in a block in cell division. CdbA binds multiple sites across the M. xanthus genome with moderate sequence specificity; however, its depletion only caused minor changes in transcription. C-di-GMP binding and DNA binding by CdbA are mutually exclusive and substitutions in the CdbA interfaces important for c-di-GMP binding not only abolished c-di-GMP binding but also DNA binding and rendered the mutant proteins non-functional in vivo. Our data are consistent with a model whereby CdbA functions as a nucleoid associated protein to organize the M. xanthus chromosome and the function of CdbA is modulated by c-di-GMP, thus, for the first time, establishing a link between c-di-GMP and chromosome organization and segregation.

2020-04-16 | PXD018028 | Pride

Myxococcus xanthus strain:DK1622

Project description:Myxococcus xanthus DK1622 transcriptome

| PRJNA493545 | ENA

Myxococcus xanthus

Project description:Global profiling of developmental gene expression in Myxococcus xanthus

| PRJNA103249 | ENA

Global profiling of gene expression in the ΔdigR and ΔsgmT mutant of Myxococcus xanthus

Project description:In Myxococcus xanthus 55% of the more than 250 two-component signal transduction systems (TCS) genes are orphan. We hypothesized that the histidine kinase SgmT and the response regulator DigR, which comprises a DNA binding domain of the HTH_Xer type, function together to regulate gene expression. We performed genome-wide expression profiling experiments to determine wether the same set of genes are differentially expressed in the ΔdigR and ΔsgmT mutants.

2012-05-18 | GSE34408 | GEO

Myxococcus xanthus DZ2

Project description:Myxococcus xanthus DZ2 Transcriptome or Gene expression

| PRJNA183539 | ENA