Activation of a novel ULK1-MLK3-ERK5 pathway by the IFNγ receptor is required for IFNγ-inducible antiviral responses

ABSTRACT: We provide evidence that Unc-51-like kinase 1 (ULK1) is activated during engagement of the Type II IFN receptor and is required for IFNγ-mediated transcription of key interferon stimulated genes involved in anti-viral and innate immune responses. Overall design: Ulk1/2+/+ and Ulk1/2-/- mouse embryonic fibroblasts (MEFs) were plated in triplicate and were left untreated or were treated for 6 hours with 2.5x10^3 IU/ml of mouse IFNγ, followed by total RNA isolation. Total mRNA profiles of untreated versus IFNγ-treated Ulk1/2+/+ and Ulk1/2-/- MEFs were then generated by deep sequencing, in triplicate, using Illumina NextSeq 500.

ORGANISM(S): Mus Musculus

PROVIDER: GSE102755 | GEO |

REPOSITORIES: GEO