Transcriptomics,Genomics

Dataset Information

156

A molecular roadmap of the aorta-gonad-mesonephros region reveals BMPER as a novel regulator of HSC maturation [AGM]


ABSTRACT: In the developing embryo, haematopoietic stem cells (HSCs) emerge from the aorta-gonad-mesonephros (AGM) region but the molecular regulation of this process is poorly understood. Recently, the progression from E9.5 to E10.5 and polarity along the dorso-ventral axis have been identified as clear demarcations of the supportive HSC niche. To identify novel secreted regulators of HSC maturation, we performed RNA-sequencing over these spatio-temporal transitions in the AGM region, and supportive OP9 cell line. Overall design: RNA-sequencing profiles of the aorta-gonad-mesonephros region from E9.5 embryos and E10.5 embryos sub-dissected into dorsal (AoD), ventral (AoV) and urogenital ridges (UGR) and pooled from between 15 and 34 embryos in three separate experiments.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Alexander Medvinsky 

PROVIDER: GSE102857 | GEO | 2017-10-30

SECONDARY ACCESSION(S): PRJNA399129

REPOSITORIES: GEO

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Publications

A molecular roadmap of the AGM region reveals BMPER as a novel regulator of HSC maturation.

McGarvey Alison C AC   Rybtsov Stanislav S   Souilhol Céline C   Tamagno Sara S   Rice Ritva R   Hills David D   Godwin Duncan D   Rice David D   Tomlinson Simon R SR   Medvinsky Alexander A  

The Journal of experimental medicine 20171101 12


In the developing embryo, hematopoietic stem cells (HSCs) emerge from the aorta-gonad-mesonephros (AGM) region, but the molecular regulation of this process is poorly understood. Recently, the progression from E9.5 to E10.5 and polarity along the dorso-ventral axis have been identified as clear demarcations of the supportive HSC niche. To identify novel secreted regulators of HSC maturation, we performed RNA sequencing over these spatiotemporal transitions in the AGM region and supportive OP9 ce  ...[more]

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