Transcriptomics,Genomics

Dataset Information

157

A molecular roadmap of the aorta-gonad-mesonephros region reveals BMPER as a novel regulator of HSC maturation [OP9]


ABSTRACT: In the developing embryo, haematopoietic stem cells (HSCs) emerge from the aorta-gonad-mesonephros (AGM) region but the molecular regulation of this process is poorly understood. Recently, the progression from E9.5 to E10.5 and polarity along the dorso-ventral axis have been identified as clear demarcations of the supportive HSC niche. To identify novel secreted regulators of HSC maturation, we performed RNA-sequencing over these spatio-temporal transitions in the AGM region, and supportive OP9 cell line. Overall design: RNA-sequencing profiles of OP9 cells grown in flat, submersed culture or reaggregate and cultured at the liquid-gas interface were compared.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Alexander Medvinsky 

PROVIDER: GSE102858 | GEO | 2017-10-30

SECONDARY ACCESSION(S): PRJNA399128

REPOSITORIES: GEO

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Publications

A molecular roadmap of the AGM region reveals BMPER as a novel regulator of HSC maturation.

McGarvey Alison C AC   Rybtsov Stanislav S   Souilhol Céline C   Tamagno Sara S   Rice Ritva R   Hills David D   Godwin Duncan D   Rice David D   Tomlinson Simon R SR   Medvinsky Alexander A  

The Journal of experimental medicine 20171101 12


In the developing embryo, hematopoietic stem cells (HSCs) emerge from the aorta-gonad-mesonephros (AGM) region, but the molecular regulation of this process is poorly understood. Recently, the progression from E9.5 to E10.5 and polarity along the dorso-ventral axis have been identified as clear demarcations of the supportive HSC niche. To identify novel secreted regulators of HSC maturation, we performed RNA sequencing over these spatiotemporal transitions in the AGM region and supportive OP9 ce  ...[more]

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