Transcriptomics,Genomics

Dataset Information

49

Myocardial reparative functions of exosomes from mouse mesenchymal stem cells (MSCs) are enhanced by hypoxia treatment


ABSTRACT: To determine whether hypoxia augments the activity of exosomes by modulating miRNAs, we use miRNA array profile to find the differential expression of miRNAs of exosomes in different culture conditions (normoxia, hypoxia, hypoxia supplemented with GW4869 which is an inhibitor of exosomes generation). Overall design: Three samples were processed for each kind of exosome that derived from normoxic-MSCs, hypoxic-MSCs, and hypoxic-MSCs suppled with GW4869 (N-EXO, H-EXO, H+G-EXO). H-Exosome was isolated from conditioned medium of MSCs under hypoxia condtion 24h. The fragmentation mixtures were hybridized to an Agilent- Mouse microRNA array 21.0

INSTRUMENT(S): Agilent-070155 Mouse miRNA Microarray (miRBase Release 21.0, miRNA ID version)

SUBMITTER: Jin Yun Zhu  

PROVIDER: GSE102912 | GEO | 2018-01-01

SECONDARY ACCESSION(S): PRJNA399263

REPOSITORIES: GEO

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Publications

Myocardial reparative functions of exosomes from mesenchymal stem cells are enhanced by hypoxia treatment of the cells via transferring microRNA-210 in an nSMase2-dependent way.

Zhu Jinyun J   Lu Kai K   Zhang Ning N   Zhao Yun Y   Ma Qunchao Q   Shen Jian J   Lin Yinuo Y   Xiang Pingping P   Tang Yaoliang Y   Hu Xinyang X   Chen Jinghai J   Zhu Wei W   Webster Keith A KA   Wang Jian'an J   Yu Hong H  

Artificial cells, nanomedicine, and biotechnology 20171116 8


Hypoxia treatment enhances paracrine effect of mesenchymal stem cells (MSCs). The aim of this study was to investigate whether exosomes from hypoxia-treated MSCs (ExoH) are superior to those from normoxia-treated MSCs (ExoN) for myocardial repair. Mouse bone marrow-derived MSCs were cultured under hypoxia or normoxia for 24 h, and exosomes from conditioned media were intramyocardially injected into infarcted heart of C57BL/6 mouse. ExoH resulted in significantly higher survival, smaller scar siz  ...[more]

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