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Half-lives mRNA analysis of Stenotrophomonas maltophilia D457 and rng-deficient mutant

ABSTRACT: We calculated half-life values of mRNAs quantified by RNA-Seq by a suitable method of normalization. We determined the half-lives of more than 2200 mRNAs in the Stenotrophomonas maltophilia D457 wild-type strain and in an isogenic RNase G deficient mutant. Median half-lives were 2,74 and 3 min in the wild-type and the rng-deficient mutant respectively. We found an overall enhancement of half-life times of mRNAs when the gene encoding RNase G is lacking, showing that many RNAs are targets of RNase G in S. maltophilia. For achieving such goal, we propose a method for the normalization of RNA-Seq based studies on global bacterial mRNA decay.

ORGANISM(S): Stenotrophomonas maltophilia

PROVIDER: GSE103467 | GEO | 2017/09/06

SECONDARY ACCESSION(S): PRJNA401735

REPOSITORIES: GEO

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