Whole genome bisulphite sequencing using the Illumina X system [Illumina HumanMethylation450 BeadChip]

ABSTRACT: While Illumina X technology has helped to reduce the cost of whole genome sequencing substantially, its application for bisulphite sequencing is not straightforward. We describe the optimization of a library preparation and sequencing approach that maximizes the yield and quality of sequencing, and how to eliminate a previously unrecognized artefact affecting several percent of bisulphite sequencing reads.

ORGANISM(S): Homo sapiens

PROVIDER: GSE103502 | GEO | 2018/07/12

REPOSITORIES: GEO