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Omics score: 173

Single cell analysis reveals cancer cell heterogeneities in hepatocellular carcinoma [SMART-seq]

ABSTRACT: Combined transcriptomic and functional analyses of HCC cells at single-cell level were performed to assess CSC heterogeneity. Overall design: Single-cell transcriptome analyses of two HCC cell lines (HuH-1 and HuH-7) and one patient-derived circulating tumor cells by using SMART-Seq protocol ***Due to patient privacy concerns, the submitter declares that patient data will be submitted to dbGaP.***

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

SUBMITTER: Xin Wei Wang

PROVIDER: GSE103866 | GEO | 2018-06-11

REPOSITORIES: GEO

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Single-cell analysis reveals cancer stem cell heterogeneity in hepatocellular carcinoma.

Zheng Hongping H Pomyen Yotsawat Y Hernandez Maria Olga MO Li Caiyi C Livak Ferenc F Tang Wei W Dang Hien H Greten Tim F TF Davis Jeremy L JL Zhao Yongmei Y Mehta Monika M Levin Yelena Y Shetty Jyoti J Tran Bao B Budhu Anuradha A Wang Xin Wei XW

Hepatology (Baltimore, Md.) 20180509 1

Intratumor molecular heterogeneity of hepatocellular carcinoma is partly attributed to the presence of hepatic cancer stem cells (CSCs). Different CSC populations defined by various cell surface markers may contain different oncogenic drivers, posing a challenge in defining molecularly targeted therapeutics. We combined transcriptomic and functional analyses of hepatocellular carcinoma cells at the single-cell level to assess the degree of CSC heterogeneity. We provide evidence that hepatic CSCs ...[more]

PMID: 29315726

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