Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of a novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE103897 | GEO | 2018/01/01

SECONDARY ACCESSION(S): PRJNA407427

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Characterization of a novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient [Genotype]

Project description:As organ-specific models of breast cancer bone metastasis do not exist, we established a novel breast cancer line was established from the ER-/PR-/HER2- bone metastasis from a breast cancer patient. This cell line was characterized and compared to the primary tumour and/or normal mammary epithelial cells with regards to phenotypes (i.e. marker expression), gene expression and copy number variation.

2018-01-01 | GSE103896 | GEO

Characterization of a novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient [gene expression]

2018-02-23 | GSE103357 | GEO

Gene expression patterns of a novel breast cancer cell line derived from a bone metastatic lesion of a breast cancer patient

2021-01-31 | GSE55715 | GEO

Characterization of a novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient

Project description:Characterization of a novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient

| PRJNA407427 | ENA

A novel breast cancer cell line derived from a metastatic bone lesion of a breast cancer patient

Project description:Purpose: We sought to generate and characterize a novel cell line from a breast cancer bone metastasis in order to better study the progression of the disease.Methods: The cell line, P7731, was derived from a metastatic bone lesion of a breast cancer patient and assessed for marker expression. P7731 was analyzed for DNA copy number variation, somatic mutations and gene expression and compared to the primary tumor. Results: P7731 cells are negative for estrogen receptor alpha (ERα), progesterone receptor (PR) and HER2 (triple-negative), strongly express vimentin (100% of cells positive) and also express cytokeratins 8/18 and 19 but at lower frequencies. Flow cytometry indicates P7731 cells are predominantly CD44+/CD49f+/EpCAM-, consistent with a primitive, mesenchymal-like phenotype. The cell line is tumorigenic in immunocompromised mice. Exome sequencing identified a total of 45 and 76 somatic mutations in the primary tumor and cell line respectively, of which 32 were identified in both samples and included mutations in known driver genes PIK3CA, TP53 and ARID1A. P7731 retains the DNA copy number alterations present in the matching primary tumor. Homozygous deletions detected in the cell line and in the primary tumor were found in regions containing three known (CDKN2A, CDKN2B and CDKN1B) and 23 putative tumor suppressor genes. Cell line specific gene amplification coupled with mRNA expression analysis revealed genes and pathways with potential pro-metastatic functions.Conclusion: This novel human breast cancer-bone metastasis cell line will be a useful model to study aspects of breast cancer biology, particularly metastasis-related changes from breast to bone.

| EGAS00001002840 | EGA