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Single-cell RNA-seq (Drop-seq) of MGE, CGE and LGE of E13.5 (MGE) and E14.5 (CGE, LGE) mouse embryos

ABSTRACT: Single-cell RNA-seq to profile a developmental time course of interneuron development, measuring the transcriptomes of progenitors during their maturation in the ganglionic eminences.

ORGANISM(S): Mus musculus

PROVIDER: GSE103983 | GEO | 2018/03/05

REPOSITORIES: GEO

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Single-cell RNA-seq (10X Genomics Chromium) of MGE, CGE, cortical and subcortical regions of E13.5, E18.5 and P10 mice

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Single-cell RNA-seq (Drop-seq) of MGE, CGE and LGE of E13.5 (MGE) and E14.5 (CGE, LGE) mouse embryos

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Project description:The homeobox containing gene Arx is expressed during ventral telencephalon development and it is required for correct GABAergic interneuron tangential migration from the ganglionic eminences to the olfactory bulbs, cerebral cortex and striatum. Its human ortholog is associated with a variety of neurological clinical manifestations whose syntoms are compatible with a loss of cortical interneurons and altered basal ganglia related-activities in humans. Herein, we reported the identification by global expression profiling of a group of genes whose expression is consistently altered in Arx mutant ganglionic eminences. Following analysis revealed the striking ectopic expression in the ganglionic eminences of a number of genes normally not, or only marginally, expressed in the ventral telencephalon. Among them, we functionally analyzed Ebf3, whose ectopic expression in ventral telencephalon is preventingneuronal tangential migration. Further, we showed that Arx is sufficient to repress Ebf3 endogenous expression and that its silencing in Arx mutant tissue might marginally rescue tangential cell movements. Together, these data provide an initial analysis of the molecular pathways regulated by Arx and how their networking might regulate those specific cellular processes during telencephalon development strongly altered by loss of Arx. Three timed-pregnant Arx heterozygous dams crossed with C57Bl/6 males were sacrificed at E14.5, the embryos harvested and placed into cold PBS. After brain isolarion, meninges and olfactory bulbs were removed, and the ventral telencephalon separated from the overlying cerebral cortex. The same procedure was repeated for 5 wt and 5 Arx mutant embryos.

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MGE, CGE, LGE and cortical regions from mouse embryonic forebrain

Project description:This SuperSeries is composed of the SubSeries listed below.

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