Genomics

Dataset Information

97

BCR heavy and light chain repertoire sequencing of pertitoneal cavity B-1a cells from wild-type and Igll1 knockout mice


ABSTRACT: We report that B-1a cells develop in a surrogate light chain independent context. As a consequence, the precursor B-1a cell population avoids a pre-BCR positive selection stage. To confirm that the B-1a cells generated in this manner repersent a bonafide B-1a cell compartment, we did NGS on BCR rearrangements to assess the repertoire diversity. We find that as a whoile, B-1a cell repertoire that develop in Igll1 kncokout mice are similar compared to wild-type. This supports our findings that B-1a cells develop properly in the absence of surrogate light chain. Overall design: Peritoneal cavity B-1a cells were sorted from 6-8 week old mice, DNA was extracted and rearranged heavy chains were amplified by PCR. These PCR products were then process to form DNA libraries that were compatible with Illumina NGS.

INSTRUMENT(S): Illumina MiSeq (Mus musculus)

SUBMITTER: Jane Skok  

PROVIDER: GSE104111 | GEO | 2018-01-15

SECONDARY ACCESSION(S): PRJNA408276

REPOSITORIES: GEO

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