Genomics

Dataset Information

45

A three–microRNA signature as a potential biomarker for the early detection of oral cancer


ABSTRACT: Background: Oral squamous cell carcinoma (OSCC) is often diagnosed at a late stage and may be malignantly transformed from oral leukoplakia (OL). This study aimed to identify potential plasma microRNAs (miRNAs) for the early detection of oral cancer. Methods: Plasma from normal, OL, and OSCC patients were evaluated. Small RNA sequencing was used to screen differently expressed miRNAs among the groups. Next, these miRNAs were validated with individual samples by quantitative real-time polymerase chain reaction (qRT-PCR) assays. The possible physiological roles of the identified miRNAs were further investigated using bioinformatics analysis. Results: Three miRNAs (miR-222-3p, miR-150-5p, and miR-423-5p) were identified as differentially expressed among groups; miR-222-3p and miR-423-5p negatively correlated with T stage, lymph node metastasis status, and clinical stage. A high diagnostic accuracy (Area under curve = 0.88) was demonstrated for discriminating OL from OSCC. Bioinformatics analysis reveals that miR-423-5p and miR-222-3p are significantly over-expressed in oral cancer tissues and involved in various cancer pathways. Conclusions: The three plasma miRNA panel may be useful to monitor malignant progression from OL to OSCC and as potential biomarkers for early detection of oral cancer. Overall design: Small RNA-seq analysis was performed on three different pooled samples (normal, oral leukoplakia and oral squmaous cell carcinoma) to identify differentially expressed miRNA.

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

SUBMITTER: Hsien-Da Huang  

PROVIDER: GSE104440 | GEO | 2018-04-17

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE104440_RAWCOUNT_MATRIX.txt.gz Txt
Items per page:
1 - 1 of 1
altmetric image

Publications

A Three-MicroRNA Signature as a Potential Biomarker for the Early Detection of Oral Cancer.

Chang Yi-An YA   Weng Shun-Long SL   Yang Shun-Fa SF   Chou Chih-Hung CH   Huang Wei-Chih WC   Tu Siang-Jyun SJ   Chang Tzu-Hao TH   Huang Chien-Ning CN   Jong Yuh-Jyh YJ   Huang Hsien-Da HD  

International journal of molecular sciences 20180307 3


Oral squamous cell carcinoma (OSCC) is often diagnosed at a late stage and may be malignantly transformed from oral leukoplakia (OL). This study aimed to identify potential plasma microRNAs (miRNAs) for the early detection of oral cancer. Plasma from normal, OL, and OSCC patients were evaluated. Small RNA sequencing was used to screen the differently expressed miRNAs among the groups. Next, these miRNAs were validated with individual samples by quantitative real-time polymerase chain reaction (q  ...[more]

Similar Datasets

2018-01-01 | S-EPMC5877619 | BioStudies
2021-01-01 | S-EPMC7806472 | BioStudies
2019-01-01 | S-EPMC6821846 | BioStudies
2018-01-01 | S-EPMC6129488 | BioStudies
2016-01-01 | S-EPMC4969402 | BioStudies
2017-01-01 | S-EPMC5352394 | BioStudies
2013-01-01 | S-EPMC3691281 | BioStudies
2020-01-01 | S-EPMC7832584 | BioStudies
1000-01-01 | S-EPMC5871085 | BioStudies
2015-01-01 | S-EPMC4642962 | BioStudies