Genomics

Dataset Information

156

Poly(A) Tail Length of L4 C. elegans


ABSTRACT: Poly(A) tails are important elements in mRNA translation and stability. However, recent genome-wide studies concluded that poly(A) tail length was generally not associated with translational efficiency in non-embryonic cells. To investigate if poly(A) tail size might be coupled to gene expression in an intact organism, we used an adapted TAIL-seq protocol to measure poly(A) tails in Caenorhabditis elegans. Surprisingly, we found that well-expressed transcripts contain relatively short, well-defined tails. This attribute appears dependent on translational efficiency, as transcripts enriched for optimal codons and ribosome association had the shortest tail sizes, while non-coding RNAs retained long tails. Overall design: Global investigation of L4 C. elegans poly(A) tail length by using mTAIL-seq and analysis by the tailseeker2 program. Two independent biological replicates were used.

INSTRUMENT(S): Illumina MiSeq (Caenorhabditis elegans)

SUBMITTER: Amy Pasquinelli  

PROVIDER: GSE104501 | GEO | 2017-10-19

SECONDARY ACCESSION(S): PRJNA412860

REPOSITORIES: GEO

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Publications

Short poly(A) tails are a conserved feature of highly expressed genes.

Lima Sarah Azoubel SA   Chipman Laura B LB   Nicholson Angela L AL   Chen Ying-Hsin YH   Yee Brian A BA   Yeo Gene W GW   Coller Jeff J   Pasquinelli Amy E AE  

Nature structural & molecular biology 20171106 12


Poly(A) tails are important elements in mRNA translation and stability, although recent genome-wide studies have concluded that poly(A) tail length is generally not associated with translational efficiency in nonembryonic cells. To investigate whether poly(A) tail size might be coupled to gene expression in an intact organism, we used an adapted TAIL-seq protocol to measure poly(A) tails in Caenorhabditis elegans. Surprisingly, we found that well-expressed transcripts contain relatively short, w  ...[more]

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