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Characterizing the transcriptional profile of murine 3T3-L1 adipocytes with altered expression of Dnmt3a

ABSTRACT: We report RNA-Seq data file from 3T3-L1 adipocytes following overexpression and knock-down of Dnmt3a

ORGANISM(S): Mus musculus

PROVIDER: GSE104508 | GEO | 2017/10/03

SECONDARY ACCESSION(S): PRJNA412870

REPOSITORIES: GEO

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Characterizing the transcriptional profile of murine 3T3-L1 adipocytes with altered expression of Dnmt3a

Project description:Characterizing the transcriptional profile of murine 3T3-L1 adipocytes with altered expression of Dnmt3a

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Glucocorticoid target genes in 3T3-L1 adipocytes

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Characterising the transcriptional profile of murine 3T3-L1 adipocytes with altered expression of IRF3.

Project description:The chronic inflammatory state that accompanies obesity is a major contributor to insulin resistance and other metabolic dysfunction features. Despite recent advances in our understanding of the cellular and secreted factors that promote the inflammatory milieu of obesity, we have much less insight into the transcriptional pathways that drive these processes. While most attention has focused on the canonical inflammatory transcription factor NF-KB, other potentially important factors exist, including members of the interferon regultory factor (IRF) family. Here we show that IRF3 expression is upregulated in the adipocytes of obese mice and humans. TLR3/TLR4 activation induces insulin resistance in adipocytes, which can be prevented by IRF3 knockdown. Furthermore, Irf3KO mice display improved insulin sensitivity, associated with reduced intra-adipose and systemic inflammation in the high-fat fed state, enhanced browning of subcutaneous fat, and increased adipose expression of Glut4. Taken together, our data indicates that IRF3 is a major transcriptional regulator of adipose inflammation to maintain systemic glucose and energy homeostasis. Transcriptional profiling of murine 3T3-L1 adipocytes with altered expression of IRF3. Overexpression or knockdown of IRF3 was achieved by lentivirus transduction for 6 days. 3T3-L1 adipocytes with IRF3 knockdown were further treated in the absence or presence of LPS for 6 days. Samples consist of triplicate replica with appropriate control.

2016-05-31 | E-GEOD-81193 | biostudies-arrayexpress

Whole genome bisulfite sequencing of 3T3-L1 preadipocytes and adipocytes

Project description:Examination of 2 samples derived from 3T3-L1 preadipocytes and adipocytes

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Gene expression signatures in IRF7 overexpressed 3T3-L1 adipocytes

Project description:To identify the genes that are regulated by IRF7, we have performed DNA microarray in 3T3-L1 adipocytes differentiated from precursor cells infected with retrovirus empty or carrying IRF7. Plasmids (Empty- and IRF7-pMSCV) were kindly provided from Dr. Eguchi at the Okayama University Graduate School of Medicine, Okayama, Japan [Cell Metab. 2008;7: 86-94.]. Infected 3T3-L1 preadipocytes were selected by puromycin treatment and differentiated into adipocytes. 7 days after the induction of adipogenesis, total RNA was isolated.

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Glucocorticoid target genes in 3T3-L1 adipocytes

Project description:Analysis of 3T3-L1 adipocytes treeated with dexamethasone (Dex) for 6 hours. Dex is a synthetic glucorticoid (GC) receptor agonist. Results provide insight into the effect of glucocorticoids on adipocytes. Differentiated 3T3-L1 adipocytes were cultured in DMEM supplemented with 5% stripped FBS for 24 hours and then treated with 500 nΜ Dex or an equal volume (0.05% v/v of media) of ethanol as a vehicle control for 6 hours at 37°C in DMEM with 190 5% stripped FBS. Total cellular RNA was isolated utilizing the NucleoSpin RNA II kit (Macherey-Nagel).RNA isolates were first quantified by standard spectrophotometry, and then qualitatively evaluated by capillary electrophoresis employing the Bio-Rad Experion system per the manufacturer’s instruction. The final labeled cRNA samples were hybridized overnight to Illumina mouseWG-6 BeadChip arrays, which was performed at UCSF Genomic Core. All treatments were done in triplicates and the same batch of microarrays were used for all treatments. The Illumina expression arrays were pre-processed using lumi package. The differential expression analysis was performed using the Limma package.

2010-09-15 | E-GEOD-24105 | biostudies-arrayexpress

Expression data from mature 3T3-L1 adipocytes under berberine treatment

Project description:We used microarrays to detail the global program of gene expression under berberine treatment in 3T3-L1 adipocytes

2014-12-31 | GSE56440 | GEO

RNAi knockdown of Tip60 and Usp7 in mouse 3T3-L1 adipocytes

Project description:Knockdown of Tip60 or Usp7 in differentiated 3T3-L1 adipocytes

2013-08-28 | E-TABM-1145 | biostudies-arrayexpress

3T3-L1: GDE5 Knockdown

Project description:Transcriptional profiling of mouse 3T3-L1 adipocytes. The objective of this study is to explore gene expression profiles of 3T3-L1 adipocytes in response to GDE5 siRNA transfection.

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