Genomics

Dataset Information

157

Polycomb Repressive Complex 2 methylates Elongin A to tune transcript levels of targeted genes [ChIP-seq]


ABSTRACT: Polycomb repressive complex 2 (PRC2-EZH2) methylates histone H3 at lysine 27 (H3K27). PRC2 is required to maintain gene repression during development and differentiation and misregulation of PRC2 is linked to a range of neoplastic malignancies, activities that are believed to involve H3K27 methylation. The full spectrum of non-histone substrates of PRC2, however, is not known, and it is not known which other substrates might also contribute to the biological functions of PRC2. We characterized the target recognition specificity and substrate diversity of PRC2, and identified more than one hundred potential novel nuclear targets of PRC2. The RNA polymerase II (Pol II) transcription elongation factor, Elongin A (EloA), is an in vivo target of PRC2. Mutation of the EloA residue that is methylated by PRC2 decreases repression of numerous PRC2 target genes. We propose that this functional crosstalk between PRC2 and EloA tunes the level of repression of targeted genes and contributes to the biological functions of PRC2. Overall design: We examined the genome-wide distribution of PRC2 catalytic subunit, EZH2, in mES cell by ChIP-seq

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Behfar Ardehali  

PROVIDER: GSE104659 | GEO | 2017-11-16

SECONDARY ACCESSION(S): PRJNA413411

REPOSITORIES: GEO

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Publications

Polycomb Repressive Complex 2 Methylates Elongin A to Regulate Transcription.

Ardehali M Behfar MB   Anselmo Anthony A   Cochrane Jesse C JC   Kundu Sharmistha S   Sadreyev Ruslan I RI   Kingston Robert E RE  

Molecular cell 20171116 5


Polycomb repressive complex 2 (PRC2-EZH2) methylates histone H3 at lysine 27 (H3K27) and is required to maintain gene repression during development. Misregulation of PRC2 is linked to a range of neoplastic malignancies, which is believed to involve methylation of H3K27. However, the full spectrum of non-histone substrates of PRC2 that might also contribute to PRC2 function is not known. We characterized the target recognition specificity of the PRC2 active site and used the resultant data to scr  ...[more]

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