Transcriptomics,Genomics

Dataset Information

45

Time course progression of BMP4-treated hESC gene expressions under 4 % and 20 % oxygen conditions.


ABSTRACT: H1 hESC were exposed to BMP4 (10 ng/ml) in absence of FGF2 for 0, 3, 12, 24, 72, and 120 h under both physiological (4 % O2) and atmospheric (20 % O2) conditions to identify candidate genes and gene networks associated with specification from totipotent precursor cells to trophoblast. RNA from cells under both conditions was isolated at each time point. Approximately 32,000 probe sets (out of 40,391) provided a signal above background at one of the time points. Of these, 14,478 showed a ≥2-fold (plus or minus) change (p<0.05) under 4 % O2, and 18,580 under 20 % O2. The data indicate the potential usefulness of hESC grown in presence of BMP4 and absence of FGF2 for studying both the emergence of trophoblast from hESC precursors and for its subsequent differentiation over time, space and varied O2 concentrations. Keywords: Genes and gene networks associated with specification from totipotent precursor cells to trophoblast Overall design: Twelve sets of H1 hESC RNA were prepared from BMP4 treated cells for 0, 3, 12, 24, 72, and 120 h under both physiological (4 % O2) and atmospheric (20 % O2) and cultured on Matrigel coated six-well plate.

INSTRUMENT(S): Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)

SUBMITTER: Toshihiko Ezashi  

PROVIDER: GSE10469 | GEO | 2009-05-10

SECONDARY ACCESSION(S): PRJNA108029

REPOSITORIES: GEO

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Publications

Derivation of induced pluripotent stem cells from pig somatic cells.

Ezashi Toshihiko T   Telugu Bhanu Prakash V L BP   Alexenko Andrei P AP   Sachdev Shrikesh S   Sinha Sunilima S   Roberts R Michael RM  

Proceedings of the National Academy of Sciences of the United States of America 20090618 27


For reasons that are unclear the production of embryonic stem cells from ungulates has proved elusive. Here, we describe induced pluripotent stem cells (iPSC) derived from porcine fetal fibroblasts by lentiviral transduction of 4 human (h) genes, hOCT4, hSOX2, hKLF4, and hc-MYC, the combination commonly used to create iPSC in mouse and human. Cells were cultured on irradiated mouse embryonic fibroblasts (MEF) and in medium supplemented with knockout serum replacement and FGF2. Compact colonies o  ...[more]

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