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Development and characterization of a mouse epithelial cell monolayer platform (APECCS) of the small and large intestines

ABSTRACT: We describe the development and characterization of a mouse epithelial cell monolayer platform of the small and large intestines with a broad range of potential applications including the discovery and development of minimally systemic drug candidates. Culture conditions for each intestinal segment were optimized by correlating monolayer global gene expression with the corresponding tissue segment. The monolayers polarized, formed tight junctions, and contained a diversity of intestinal epithelial cell lineages.

ORGANISM(S): Mus musculus

PROVIDER: GSE104803 | GEO | 2017/11/30

SECONDARY ACCESSION(S): PRJNA413886

REPOSITORIES: GEO

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