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An OMIC approach to elaborate the antibacterial mechanisms of different alkaloids

ABSTRACT: In the current study, RNA sequencing was used to comparatively elaborate the activities and the effects of the alkaloids, boldine, bulbocapnine, and roemerine along with the well-known antibacterial alkaloid berberine on Bacillus subtilis cells.

ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168

PROVIDER: GSE104946 | GEO | 2018/07/17

REPOSITORIES: GEO

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An OMIC approach to elaborate the antibacterial mechanisms of different alkaloids

Project description:An OMIC approach to elaborate the antibacterial mechanisms of different alkaloids

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Potentiating the activity of berberine for Staphylococcus aureus in a combinatorial treatment with thymol

Project description:In the current study, RNA sequencing based transcriptional profiling was used to elaborate the combinatorial effect of berberine, a well-known antibacterial alkaloid, and thymol, a monoterpenic phenol, on the Staphylococcus aureus transcriptome.

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Repurposing bioactive aporphine alkaloids as efflux pump inhibitors

Project description:In the current study, RNA sequencing was used to investigate the synergistic effects of the berberine and roemerine on Bacillus subtilis cells.

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Alkaloids modify the poison frog proteome

Project description:Poison frogs sequester chemical defenses from their diet of leaf litter arthropods for defense against predation. Little is known about the physiological adaptations that confer this unusual bioaccumulation ability. We conducted an alkaloid-feeding experiment with the Diablito poison frog (Oophaga sylvatica) to determine how quickly alkaloids are accumulated and how toxins modify frog physiology using quantitative proteomics. Diablito frogs rapidly accumulated the alkaloid decahydroquinoline within four days, and dietary alkaloid exposure modified protein abundance in the intestines, liver, and skin. Many proteins that increased in abundance with toxin accumulation are plasma glycoproteins, including the complement system and the toxin-binding protein saxiphilin. Other protein classes that change in abundance with toxin accumulation are membrane proteins involved in small molecule transport and metabolism. Overall, this work shows poison frogs can rapidly accumulate alkaloids, which alter carrier protein abundance, initiate an immune response, and alter small molecule transport and metabolism dynamics across tissues

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Transcriptional Profile of the Shigella flexneri Response to Berberine

Project description:Berberine is a natural isoquinoline alkaloid found in Chinese medicinal herbs which is active against a variety of microbial infections. To examine the potential effects of berberine on Shigella flexneri, a whole-genome DNA microarray was constructed and transcriptome analysis of the cellular responses of S.flexneri when exposed to Berberine Chloride (BC) was performed.

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Genome-wide analysis of berberine chloride-responsive gene expression in HepG2 liver cell line

Project description:Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects. Here, we used gene expression microarray to analyze gene expression profiles of HepG2 human hepatoma cell line after berberine chloride treatment or 0.08% DMSO as control. Comparing gene expression profiles of 40 M-BM-5M-BM--M berberine-treated HepG2 human hepatoma cell line to those of control cells sampled after 4 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 M-BM-5M-BM--M berberine chloride or 0.08% DMSO as control.

2013-10-02 | E-GEOD-47786 | biostudies-arrayexpress

Genome-wide analysis of berberine chloride-responsive gene expression in HepG2 liver cell line

2013-10-02 | GSE47786 | GEO

MicroRNA expression profiling of primary human hepatocytes after berberine chloride treatment

Project description:Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects. Since xenobiotic drug-induced micoRNAs have recently emerged as key regulators in guiding their pharmacological effects and toxicity, we were interested in whether or not micoRNA expression was differentially altered by berberine treatment in liver. Here, we used miRNA microarray to analyze microRNA expression profiles of primary human hepatocytes after berberine chloride treatment or 0.08% DMSO as control. Comparing miRNA profiles of 40 ïM berberine-treated primary human hepatocytes to those of control cells sampled after 2 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 ïM berberine chloride or 0.08% DMSO as control.

2015-12-31 | E-GEOD-53413 | biostudies-arrayexpress

Gene expression profiling of primary human hepatocytes after berberine chloride treatment

Project description:Berberine, an isoquinoline alkaloid isolated from many medicinal herbs such as Coptis chinensis, has a wide range of pharmacological effects. Since xenobiotic drug-induced micoRNAs have recently emerged as key regulators in guiding their pharmacological effects and toxicity, we were interested in whether or not micoRNA expression was differentially altered by berberine treatment in liver. Here, we used miRNA microarray to analyze microRNA expression profiles of primary human hepatocytes after berberine chloride treatment or 0.08% DMSO as control. Comparing gene expression profiles of 40 ï?M berberine-treated primary human hepatocytes to those of control cells sampled after 2, 4, or 8 hours treatment. A 50 mM stock solution of Berberine chloride was prepared in DMSO. Cells were treated with 40 ï?M berberine chloride or 0.08% DMSO as control.

2015-12-31 | E-GEOD-53415 | biostudies-arrayexpress

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