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Transcriptomic analysis of CD11b+ DsRed+ CD45hi cells harvested from the ischemic brain of Selplg-KODsRed → WT chimeras and Selplg-WTDsRed→ WT chimeras at 7 days after 30 min MCAo/reperfusion

ABSTRACT: In order to assess whether PSGL-1 (SELPLG) deficiency impacts key characteristics of invading cells in our stroke model, we performed a detailed transcriptomic analysis of retrovirally transduced CD11b+ CD45hi cells harvested from the ischemic brain at 7 days after MCAo

ORGANISM(S): Mus musculus

PROVIDER: GSE105011 | GEO | 2017/12/13

SECONDARY ACCESSION(S): PRJNA414418

REPOSITORIES: GEO

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Transcriptomic analysis of CD11b+ DsRed+ CD45hi cells harvested from the ischemic brain of Selplg-KODsRed → WT chimeras and Selplg-WTDsRed→ WT chimeras at 7 days after 30 min MCAo/reperfusion

Project description:Transcriptomic analysis of CD11b+ DsRed+ CD45hi cells harvested from the ischemic brain of Selplg-KODsRed → WT chimeras and Selplg-WTDsRed→ WT chimeras at 7 days after 30 min MCAo/reperfusion

| PRJNA414418 | ENA

Gene expression profile at single cell level of OT-I CD8+ T cells from B16-OVA melanoma tumors

Project description:Tumor infiltrating T cells are a diverse and heterogeneous population. We used single cell RNA sequencing (scRNA-seq) to analyze gene expression changes in tumor-specific T cells that were sufficient for or lacked PSGL-1 (gene: Selplg).

2023-04-03 | GSE226523 | GEO

Epigenetic profiling of OT-I CD8+ T cells after T cell activation

Project description:T cells undergo epigenetic changes upon T cell activation. We used the Assay for Transposase-Accessible Chromatin sequencing (ATAC-seq) to analyze epigenetic changes in T cells that were sufficient for or lacked PSGL-1 (gene: Selplg) two hours after T cell activation.

2023-04-03 | GSE226521 | GEO

mRNA microarray in MCAo rat model with IV-hUMSC

Project description:To evaluate the change of gene expression at cerebral infarction by the treatment of IV-human umblical cord derived mesenchymal stem cell. RNA was isolated from the ipsilateral hemisphere to MCAo in rats. At 72 h post-MCAo, the ipsilateral hemisphere subjected to MCAo was used for mRNA microarray. RNA was isolated from the ipsilateral hemisphere to MCAo in rats without MCAo (control group, n=5), rats treated with 1x106 IV-hUMSC (hUMSC group, n=6) and saline (saline group, n=5) at 24h post-MCAo.

2017-03-01 | GSE78731 | GEO

Expression data from rat (Sprague Dawley) brain subjected to MCAo.

Project description:Middle cerebral artery occlusion (MCAo) in rat represent the ischemic stroke in human. Rodents subjected to MCAo and treated with venom phospholipase A2 showed reduction in infarct volume after 24hours of stroke. We studied the global gene expression of the reduction in infarct volume using Affymetrix Gene Chips. We analysed all the genes that were up or down regulated in the study. Total RNA isolated from sham, MCAo and MCAo+nPLA rat brains, was pooled to minimize inter-individual variation and hybridized to each array of the RAE-230A or U34A GeneChipTM according to protocols described in the GeneChipTM expression analysis package (Affymetrix, CA).

2009-09-04 | E-GEOD-17929 | biostudies-arrayexpress

Expression data from rat (Sprague Dawley) brain subjected to MCAo.

2009-09-04 | GSE17929 | GEO

mRNA Seqeuencing of MCAO and trat pons after stroke [bulk RNA-seq]

Project description:We report the result of mRNA-seq of MCAO and pontine infarction in rats.

2022-11-25 | GSE199066 | GEO

Brain tissue of mice:sham group, the 3d after MCAO group, the 7d after MCAO group

Project description:Brain tissue of mice:sham group, the 3d after MCAO group, the 7d after MCAO group

| PRJNA804911 | ENA

Transcription profiling of mouse forebrain with induced middle cerebral artery occlusion (MCAO) to study permanent focal cerebral ischemia

Project description:profiling gene transcription in a mouse model of permanent focal cerebral ischemia that was induced by middle cerebral artery occlusion (MCAO)

2009-03-07 | E-TABM-655 | biostudies-arrayexpress

Transcriptome analysis of virus-specific H2-Db-GP33-41+CD8+ T cells from WT or PSGL-1 KO mouse spleens at day 9 post LCMV Cl 13 infection

Project description:Chronic viruses and cancers thwart immune responses in humans by inducing T cell dysfunction. Using a murine chronic virus that models human infections, we investigated the function of the adhesion molecule, P-selectin glycoprotein ligand-1 (PSGL-1) that is upregulated on responding T cells. PSGL-1-deficient mice unexpectedly cleared the virus due to dramatic increases in the intrinsic survival of multifunctional effector T cells that had downregulated PD-1 and other inhibitory receptors. Notably, this response resulted in CD4+ T cell-dependent immunopathology. Mechanistically, PSGL-1 ligation on exhausted CD8+ T cells inhibited TCR and IL-2 signaling, and upregulated PD-1, leading to diminished survival with TCR stimulation. In models of malignant melanoma where T cell dysfunction occurs, PSGL-1-deficiency led to PD-1 downregulation, improved T cell responses, and tumor control. Thus, PSGL-1 plays a fundamental role in balancing viral control and immunopathology, and also functions as a checkpoint that regulates T cell responses in the tumor microenvironment. WT or PSGL-1 KO mice were infected with 2 x 10^6 PFU LCMV Clone 13. Spleens from 10 WT or 10 PSGL-1 KO animals were pooled and processed. CD8+ T cells were negatively enriched from WT or PSGL-1 KO spleens (EasySep Stemcell). Purified T cells were stained with propidium iodide (PI) for 10 minutes on ice, cells were washed. CD8+ T cells were stained with H2-Db-GP33-41 tetramers (NIH) and FACS sorted (BD FACS Aria). Sorted tetramer+ cells were PI negative and purity was >98%. Experiment was repeated twice to generate 2 WT (WT 1; WT 2) and 2 PSGL-1 KO (KO 1; KO 2) samples that represented 10 pooled spleens per sample.

2016-06-30 | E-GEOD-80113 | biostudies-arrayexpress

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