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Single cell chromatin immunocleavage sequencing (scChIC-Seq) to profile histone modification

ABSTRACT: We report a single-cell chromatin immunocleavage sequencing (scChIC-seq) methodology for analyzing histone modifications, which involves targeting of the micrococcal nuclease (MNase) by tethering it to an antibody and selective PCR amplification of cleaved target sites. We show that the protocol reliably detects the H3K4me3 and H3K27me3 target sites in single human white blood cells (WBC), resulting data for successful identification of unique blood cell types based on clustering analysis.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE105012 | GEO | 2019/03/28

REPOSITORIES: GEO

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ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo

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