Transcriptomics

Dataset Information

21

U937 cells: differentiating vs. undifferentiated


ABSTRACT: Gene-expression changes in U937 cells undergoing monocytic differentiation with TPA treatment Keywords: differentiation treatment Overall design: The promonocytic U937 cell line was maintained at 37C in RPMI 1640 medium (Mediatech) supplemented with 10% fetalclone (HyClone) under a 5% CO2, 95% air atmosphere. U937 cells were differentiated by the addition to the growth medium 100 nM 12-O-tetradeconoyl-phorbol 13-acetate (TPA) (Sigma).Three condition experiment, treated with TPA for two different time points (27 and 96 hrs) vs. untreated. Biological replicates: independently grown, treated and harvested

INSTRUMENT(S): University of Illinois at Chicago Human Expression 35K - Operon version 3.0

ORGANISM(S): Homo sapiens  

SUBMITTER: Elizaveta V Benevolenskaya   

PROVIDER: GSE10560 | GEO | 2008-08-29

SECONDARY ACCESSION(S): PRJNA107813

REPOSITORIES: GEO

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