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U937 cells: differentiating vs. undifferentiated

ABSTRACT: Gene-expression changes in U937 cells undergoing monocytic differentiation with TPA treatment Keywords: differentiation treatment

ORGANISM(S): Homo sapiens

PROVIDER: GSE10560 | GEO | 2008/08/29

SECONDARY ACCESSION(S): PRJNA107813

REPOSITORIES: GEO

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U937 cells: differentiating vs. undifferentiated

Project description:Gene-expression changes in U937 cells undergoing monocytic differentiation with TPA treatment Keywords: differentiation treatment The promonocytic U937 cell line was maintained at 37C in RPMI 1640 medium (Mediatech) supplemented with 10% fetalclone (HyClone) under a 5% CO2, 95% air atmosphere. U937 cells were differentiated by the addition to the growth medium 100 nM 12-O-tetradeconoyl-phorbol 13-acetate (TPA) (Sigma).Three condition experiment, treated with TPA for two different time points (27 and 96 hrs) vs. untreated. Biological replicates: independently grown, treated and harvested

2010-06-20 | E-GEOD-10560 | biostudies-arrayexpress

Induction of microRNA expression and processing via ERK/MAPK signaling during monocytic differentiation

Project description:This study produced a microarray-based screening for miRNAs responding to tetradecanoyl phorbol acetate (TPA) (for monocytic differentiation) treatment in various leukemia cell lines. Using the microarray technique, global changes in miRNA expression after treatment of four leukemia cell lines (NB4, HL-60, U937 and K562) with TPA was profiled. This microarray chip contained 924 probes for 802 miRNAs from human, mouse and rat after discarding redundant sequences, and a further 122 predicted miRNA sequences from published data. To reduce individual variability, replicate array analysis used independently treated samples, and a technical replicate was also performed with the same sample.

2013-12-01 | E-GEOD-33537 | biostudies-arrayexpress

Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (gene expression)

Project description:Since the expression profile of miRNAs is specific in different tissues or under different physiological conditions, the correlations between miRNAs and mRNAs could vary under different biological circumstances. This is a study also used expression profiles of miRNA and mRNA during monocytic differentiation to explore the correlations between the expression levels of miRNAs and mRNA, either negative or positive. After treatment of TPA, U937 cells present functional differentiation markers as well as specific cell morphology, which were confirmed by flow cytometric analysis and Wright-Giemsa staining.Here we use genome-wide microarray analysis to profile the expression levels of mRNA in normal U937 cells and differentiated U937 cells. For each differentiated and control sample, two hybridizations were performed by using a reversal fluorescent strategy.

2012-02-02 | E-GEOD-26377 | biostudies-arrayexpress

Monocytic U937 cells and receptor cross-linking

Project description:Monocytic U937 cell lines and crosslinked CD32 & CD64 at different time points Keywords: time-course

2009-07-20 | GSE1537 | GEO

Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (miRNA)

Project description:Since the expression profile of miRNAs is specific in different tissues or under different physiological conditions, the correlations between miRNAs and mRNAs could vary under different biological circumstances. This is a study also used expression profiles of miRNA and mRNA during monocytic differentiation to explore the correlations between the expression levels of miRNAs and mRNA, either negative or positive. After treatment of TPA, U937 cells present functional differentiation markers as well as specific cell morphology, which were confirmed by flow cytometric analysis and Wright-Giemsa staining.Here we use a miRNA microarray (CapitalBio, Beijing, China) containing 509 well-characterized human, mouse and rat miRNAs and various controls to profile the expression levels of miRNA in normal U937 cells and differentiated U937 cells. Three independent biological experiments were performed, and for each differentiated and control sample, two hybridizations were performed by using a reversal fluorescent strategy.

2012-02-02 | E-GEOD-26376 | biostudies-arrayexpress

U937 ctrl vs U937 Saha (Vorinostat; suberoylanilide hydroxamic acid) 6h

Project description:miRNA expression profiling of U937 ctrl vs U937 treated Saha 5 µM 6h

2017-05-22 | GSE69957 | GEO

U937 scramble (Usc) vs U937 miR-194-5p (UmiR-194-5p)

Project description:Transcriptional profiling of U937 scramble vs U937 miR-194-5p (UmiR-194-5p)

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Transcrription profiling of human monocytic U937 cells and receptor cross-linking

Project description:Monocytic U937 cell lines and crosslinked CD32 & CD64 at different time points

2009-08-07 | E-GEOD-1537 | biostudies-arrayexpress

The differentiation/retrodifferentiation program of human U937 leukemia cells is accompanied by changes of VCP/p97

Project description:A possible functional role of VCP/p97 during differentiation of U937 cells was addressed by siRNA-targeting of VCP/p97. Functional changes in VCP-siRNA-transfected cells following phorbol ester-induced monocytic differentiation have been examind by DNA microarray analysis. Cells transfected with the non-targeting AllStars negative siRNA (Qiagen) served as a reference. Computed

2010-06-29 | E-GEOD-9821 | biostudies-arrayexpress

The differentiation/retrodifferentiation program of human U937 leukemia cells is accompanied by changes of VCP/p97

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