Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin

ABSTRACT: ChIP-chip analyses of ClrC components across the S. pombe genome. Keywords: ChIP-chip

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE10561 | GEO | 2008/03/16

SECONDARY ACCESSION(S): PRJNA107815

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin

Project description:ChIP-chip analyses of ClrC components across the S. pombe genome. Keywords: ChIP-chip Chromatin immunoprecipitated DNA recovered with antibody against FLAG-tagged protein from wild-type asynchronous growing fission yeast cells and whole cell extracts DNA were amplified by random priming and respectively labeled with Cy5 and Cy3. Labeled DNA samples were mixed and hybridized onto a 44k pombe Agilent oligo array. Data were processed using Agilent scanner and Feature Extraction software

2010-05-25 | E-GEOD-10561 | biostudies-arrayexpress

Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance.

Project description: Not available

| S-EPMC3642681 | biostudies-literature

A histone H3K9M mutation traps histone methyltransferase Clr4 to prevent heterochromatin spreading.

Project description: Not available

| S-EPMC5030085 | biostudies-literature

Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs

2009-08-25 | GSE17271 | GEO

Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs

Project description:Expression profile of mutant cells compared to wild-type cells: Expression profile of Schizosaccharomyces pombe genome in ∆pht1, ∆ago1, ∆clr4, rik1, ∆pht1rik1, ∆pht1∆ago1 and ∆pht1∆clr4 cells. Expression profile of Schizosaccharomyces pombe genome in ∆rrp6, ∆cid14, ∆swi6, ∆swr1, ∆set1, ∆pht1∆swi6 and ∆pht1∆set1 cells. Expression profile of Schizosaccharomyces pombe genome in ∆alp13, ∆cph1, ∆set2, clr6-1 and ∆cph1∆pht1 cells. Occupancy profiling: Occupancy profiling of RNA polymerase II, histone variant H2A.Z and ClrC subunit Rik1 in fission yeast Schizosaccharomyces pombe Occupancy profiling of histone variant H2A.Z in ∆msc1 cells. Agilent 60mer oligonucleotide custom array containing probes spanning large portion of chromosome 2 at 50bp resolution was used to profile expression levels in mutant cells and to compare them to levels in wild-type cells. Agilent 60mer array was used to analyze DNA recovered by immunoprecipitation of RNA polymerase II, H2A.Z or Rik1 from asynchronous culture of fission yeast. Agilent 60mer array was used to analyze DNA recovered by immunoprecipitation of histone H2A.Z from asynchronous culture of fission yeast.

2009-08-25 | E-GEOD-17271 | biostudies-arrayexpress

The histone H3K9M mutation synergizes with H3K14 ubiquitylation to selectively sequester histone H3K9 methyltransferase Clr4 at heterochromatin.

Project description: Not available

| S-EPMC8167812 | biostudies-literature

Recruitment of Clr4 to heterochromatin

Project description:To investigate the role of Clr4 recruitment, we performed FLAG-Clr4 ChIP-seq in various genetic background

2024-01-01 | GSE223202 | GEO

Set3 contributes to heterochromatin integrity by promoting transcription of subunits of Clr4-Rik1-Cul4 histone methyltransferase complex in fission yeast.

Project description: Not available

| S-EPMC4990937 | biostudies-literature

Set1/COMPASS repels heterochromatin invasion at euchromatic sites by disrupting Suv39/Clr4 activity and nucleosome stability

Project description:We report global occupancy of H3K9me2 and H3K4me3 in wild-type S. pombe and two mutant backgrounds - the deletion of heterochromatin antagonist epe1 and the double mutant absent epe1 and the H3K4 methyltransferase set1

2020-08-24 | GSE140067 | GEO

Role of Swi6/HP1 self-association-mediated recruitment of Clr4/Suv39 in establishment and maintenance of heterochromatin in fission yeast.

Project description: Not available

| S-EPMC3058978 | biostudies-literature