Transcriptomics

Dataset Information

20

Comparison of gene expression in erythroblasts with the In(Lu) phenotype with normal erythroblasts.


ABSTRACT: In order to elucidate the molecular mechanism giving rise to the rare In(Lu) type of Lu(a-b-) blood group phenotype we compared the transcriptome of normal and In(Lu) erythroblasts at different stages of maturation. Many erythroid-specific genes had reduced transcript levels suggesting the phenotype resulted from a transcription factor abnormality. A search for mutations in erythroid transcription factors revealed mutations in the promoter or coding sequence of EKLF in 21 of 24 individuals with the In(Lu) phenotype. In all cases the mutant EKLF allele occurred in the presence of a normal EKLF allele. Individuals with the In(Lu) phenotype have no reported pathology indicating that one functional EKLF allele is sufficient to sustain human erythropoiesis. These data provide the first description of inactivating mutations in human EKLF and the first demonstration of a blood group phenotype resulting from mutations in a transcription factor. Keywords: Time course and cell type comparison. Overall design: Time course study looking at changes in gene expression during erythropoiesis in two cell types: cultured erythroblasts (days 4, 6 and 11) from normal blood donors and from those with the rare blood group In(Lu) phenotype. There are 20 samples in total (10 for each cell type). For each cell type there are 2 samples at day 4 (1 biological replicate, 2 technical replicates, and 4 samples at each of days 6 and 11 (2 biological replicates, 2 technical replicates).

INSTRUMENT(S): [HG-U133A] Affymetrix Human Genome U133A Array

ORGANISM(S): Homo sapiens  

SUBMITTER: Belinda Singleton  

PROVIDER: GSE10584 | GEO | 2008-08-11

SECONDARY ACCESSION(S): PRJNA107851

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2012-03-27 | E-GEOD-10584 | ArrayExpress
| GSE110936 | GEO
2012-06-26 | E-GEOD-22903 | ArrayExpress
2011-11-14 | GSE22903 | GEO
2014-04-15 | E-GEOD-53635 | ArrayExpress
| GSE54864 | GEO
| GSE98375 | GEO
2014-06-02 | E-GEOD-27893 | ArrayExpress
| GSE102056 | GEO
2014-06-02 | E-GEOD-32110 | ArrayExpress