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Gene expression in stria vascularis of mice lacking Slc26a4 and heterzygous controls before the onset of hearing.

ABSTRACT: Determination of differential expression of genes in the stria vascularis of pendrin (Slc26a4) heterozygous and knockout mice before the onset of hearing at postnatal day 10 (P10). Keywords: differential expression under disease state

ORGANISM(S): Mus musculus

PROVIDER: GSE10587 | GEO | 2009/02/19

SECONDARY ACCESSION(S): PRJNA107857

REPOSITORIES: GEO

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Transcription profiling of mouse stria vascularis from animals lacking Slc26a4 and heterzygous controls before the onset of hearing.

Project description:Determination of differential expression of genes in the stria vascularis of pendrin (Slc26a4) heterozygous and knockout mice before the onset of hearing at postnatal day 10 (P10). Experiment Overall Design: A total of Six samples of stria vascularis RNA obtained from P10 mice were analyzed. Triplicates from pendrin (Slc26a4) heterozygous and knockout mice were run and analyzed.

2009-03-01 | E-GEOD-10587 | biostudies-arrayexpress

Transcription profiling of mouse stria vascularis FROM 129S6 strain lacking slc26a4 AND wildtype controls

Project description:To see for differential expression of genes in the stria vascularis of wild type and pendrin knockout mice Experiment Overall Design: Six samples in total of stria vascularis RNA were analyzed. Triplicates from wild type and pendrin knockout mice were run and analyzed.

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Microarray based comparison of gene expression in stria vascularis of 129S6 mice lacking slc26a4 and wildtype controls

Project description:To see for differential expression of genes in the stria vascularis of wild type and pendrin knockout mice Keywords: Knockout vs Control

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Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4-/- mouse

Project description:The primary pathological alternations of SLC26A4-related hearing loss are endolymphatic pH acidification and luminal enlargement of the inner ear. However, the molecular cell type-specific influences remain poorly characterized. In this study, we confirmed a method of isolating single cells in the cochlea's stria vascularis (SV) from wild-type (WT) animals. We then applied it to characterize the distinct transcriptomes from mutant (Slc26a4+/- and Slc26a4-/-) samples. Single-cell RNA-sequencing (scRNA-seq) identified transcriptional profiles in Slc26a4-expressing (spindle) cells and Kcnj10-expressing (intermediate) cells of the SV. By Gene Set Enrichment Analysis (GSEA), we found that spindle cells contain extrinsic cellular components, a factor that enables cell-to-cell communication. In addition, the gene expression profile informed signaling pathways associated with pH regulation in spindle cells. Compared to WT control, the transcriptional profiles in the SV cells of Slc26a4-/- mice showed downregulation of extracellular exosome-related genes in spindle cells and GPI-anchor-related genes in the intermediate cells. Immunofluorescence studies in spindle cells of Slc26a4-/- mice validated the increased expression of the clathrin-mediated endocytosis-related protein. Overall, cell isolation of SV from WT and mutant (Slc26a4+/- and Slc26a4-/-) samples combined with cell type-specific transcriptomic analyses revealed pH-dependent alternations in spindle cells and intermediate cells, inspiring further studies into the dysfunctional role of SV cells in SLC26A4-related hearing loss.

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