DNase I hypersensitivity with capture in mES cells

ABSTRACT: This dataset introduces a new protocol called DNase-capture, a method that focuses DNase-seq analysis on selected genomic regions, resulting in a substantial increase in region-specific sequencing coverage. DNase-seq takes advantage of the preferential cutting of DNase I. DNase-capture enhances the resolution of DNase-seq by mixing a DNase-seq library with biotin-tagged bait RNA sequences that are complementary to regions of interest. In a hybridization reaction, target DNA is captured away from background DNA through streptavidin bead binding, and the captured target DNA is sequenced.

ORGANISM(S): Mus musculus

PROVIDER: GSE106217 | GEO | 2017/10/27

SECONDARY ACCESSION(S): PRJNA415942

REPOSITORIES: GEO