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Transfer RNA Genes Affect Chromosome Architecture and Function [RNA-seq]

ABSTRACT: In this study, we investigated the role of tranfer RNA genes (tDNAs) in genome organization and nuclear function by generating a chromosome lacking all its tDNAs. Our analyses of this tDNA-less chromosome show that loss of these genes affects nucleosome postioning, SMC protein binding, centromere clustering, long range chromosome folding and epigenetic silencing.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE106248 | GEO | 2019/03/05

REPOSITORIES: GEO

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Transfer RNA Genes Affect Chromosome Architecture and Function [ChIP-seq]

Project description:In this study, we investigated the role of tranfer RNA genes (tDNAs) in genome organization and nuclear function by generating a chromosome lacking all its tDNAs. Our analyses of this tDNA-less chromosome show that loss of these genes affects nucleosome postioning, SMC protein binding, centromere clustering, long range chromosome folding and epigenetic silencing.

2019-03-05 | GSE106249 | GEO

Transfer RNA Genes Affect Chromosome Architecture and Function

Project description:Chromosomes are packaged and organized in the nucleus in an ordered, non-random manner. This organization influences many nuclear processes such as transcription, gene silencing and mitosis. While transfer RNA genes (tDNAs) are essential for the generation of tRNAs these gene loci are also binding sites for transcription factors and chromosomal architectural proteins. In the yeast Saccharomyces cerevisiae, tDNAs are dispersed along all sixteen chromosomes. In this study, we investigated the role of tDNAs in genomic organization and nuclear function by editing a chromosome so that it lacks any tDNAs. Our analyses of this tDNA-less chromosome show that loss of tDNAs affect nucleosome positioning, binding of SMC proteins, centromere clustering, long-range chromosome folding and epigenetic gene silencing. We propose that these effects are primarily mediated via changes in local interactions between tDNAs and other regulatory sequences that then manifest as alterations in long-range chromosome architecture with effects on gene regulation over large distances.

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Genome-wide ChIP-exo profiling of Fpt1 in glucose

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DNA-loop extruding SMC complexes can traverse one another in vivo

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Transfer RNA Genes Affect Chromosome Architecture and Function [ChIP-seq]

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