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Role of Lsd1 in HOTAIR function

ABSTRACT: HOTAIR is a scafold long non-coding RNA tethering PRC2 and Lsd1/REST/coREST complexes to gene promoters to repress transcription of genes involved in epithelial to mesenchymal transition (EMT). To decipher a role of the Lsd1 in HOTAIR function we generated epithelial cell lines expressing full length and truncated versions of HOTAIR missing first 5'-300 (Epi-HOTΔP) and last 3'-500 bp (Epi-HOTΔL) interacting with HOTAIR partners. These cell lines were further used to determine Lsd1 binding sites using chromatin immunoprecipitation approach.

ORGANISM(S): Homo sapiens

PROVIDER: GSE106262 | GEO | 2021/04/22

REPOSITORIES: GEO

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Identification and functional characterization of long non-coding RNAs associated with epithelial-to-mesenchymal transition

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Role of Lsd1 in HOTAIR function

Project description:Role of Lsd1 in HOTAIR function

| PRJNA416065 | ENA

ChIP-chip of siGFP- or siHOTAIR-treated foreskin fibroblasts with anti-LSD1 or anti-SUZ12 antibodies on human HG18 Nimblegen promoter arrays

2010-07-08 | GSE22344 | GEO

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