Transcriptomics,Genomics

Dataset Information

36

MiRNA changes after overexpression of uc.372 in HepG2 cells


ABSTRACT: To identify putative miRNA targets for uc.372, we overexpressed uc.372 in HepG2 cells and carried out a microarray analysis. A total of 66 miRNAs present in the array demonstrated 1.5-fold upregulation or downregulation of expression upon uc.372 transfection. Overall design: HepG2 cells were tranfected with ad-uc.372 or ad-NC for 48 h. The RNA was collected and further applied for microarray analysis.

INSTRUMENT(S): Agilent-070156 Human miRNA [miRNA version]

SUBMITTER: Jian Li  

PROVIDER: GSE106366 | GEO | 2017-11-01

SECONDARY ACCESSION(S): PRJNA416447

REPOSITORIES: GEO

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Publications

Ultraconserved element uc.372 drives hepatic lipid accumulation by suppressing miR-195/miR4668 maturation.

Guo Jun J   Fang Weiwei W   Sun Libo L   Lu Yonggang Y   Dou Lin L   Huang Xiuqing X   Tang Weiqing W   Yu Liqing L   Li Jian J  

Nature communications 20180209 1


Ultraconserved (uc) RNAs, a class of long non-coding RNAs (lncRNAs), are conserved across humans, mice, and rats, but the physiological significance and pathological role of ucRNAs is largely unknown. Here we show that uc.372 is upregulated in the livers of db/db mice, HFD-fed mice, and NAFLD patients. Gain-of-function and loss-of-function studies indicate that uc.372 drives hepatic lipid accumulation in mice by promoting lipogenesis. We further demonstrate that uc.372 binds to pri-miR-195/pri-m  ...[more]

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