Determining mRNA half-lives on a transcriptome-wide scale

ABSTRACT: Translation and mRNA decay are intimately connected processes, and translational inhibition often precedes and stimulates transcript degradation. Here, we have focused on methods that allow determination of mRNA stability on a transcriptome-wide scale. We describe experimental and computational methods for the two most commonly used approaches (transcriptional inhibition and metabolic labeling), and we discuss associated caveats.

ORGANISM(S): Homo sapiens

PROVIDER: GSE106393 | GEO | 2017/12/15

SECONDARY ACCESSION(S): PRJNA416702

REPOSITORIES: GEO