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Primary human lung microvascular endothelial cells treated with universal interferon alpha beta or interferon gamma

ABSTRACT: The purpose is to obtain samples for mRNA analysis in primary human lung microvascular endothelial cells treated with universal interferon alpha beta or interferon gamma.

ORGANISM(S): Homo sapiens

PROVIDER: GSE106524 | GEO | 2017/12/27

SECONDARY ACCESSION(S): PRJNA417087

REPOSITORIES: GEO

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Primary human lung microvascular endothelial cells treated with universal interferon alpha beta or interferon gamma

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Project description:The purpose is to obtain samples for microRNA analysis in primary human microvascular endothelial cells infected with wild type Mers-CoV (icMERS). Human microvascular endothelial cells were infected with a multiplicity of infection of 5 PFU per cell. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 12 and 24 h post-infection. MERSCOV002.0P references the write up of the experimental procedure.

2016-04-06 | E-GEOD-79216 | biostudies-arrayexpress

Primary human microvascular endothelial cell transcriptome (mRNA) response to a wild type Mers-CoV (icMERS-CoV EMC2012)

Project description:The purpose is to obtain samples for mRNA analysis in primary human microvascular endothelial cells infected with wild type Mers-CoV (icMERS). Human microvascular endothelial cells were infected with a multiplicity of infection of 5 PFU per cell. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 12, 24, 36, and 48 h post-infection. MERSCOV002.0P references the write up of the experimental procedure.

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Transcription profiling of Toxoplasma-infected human fibroblasts treated with human recombinant interferon gamma

Project description:Human fibroblasts were infected or not with Toxoplasma gondii (Pru strain) for 18 h at a nominal MOI of 3. Cells were subsequently treated with human recombinant interferon gamma (0.5 ng/ml = 30 pM) for 0, 2, 4 and 8 h before RNA was harvested for cDNA microarray experiments. Uninfected samples were named N0, N2, N4 and N8 (i.e., N2 indicated uninfected cells treated with interferon gamma for 2 h); infected samples were named P0, P2, P4 and P8. Duplicate cultures were analyzed for each condition (indicated as "a" and "b"). The reference channel (ch1, green) contained Universal Human Reference RNA. Using a genome-wide microarray analysis we show here a complete dysregulation of interferon-gamma-inducible gene expression in human fibroblasts infected with Toxoplasma. Notably, 46 of the 127 interferon-gamma-responsive genes were induced and 19 were suppressed in infected cells before they were exposed to interferon gamma, indicating that other stimuli produced during infection may also regulate these genes. Following interferon-gamma treatment, none of the 127 interferon-gamma-responsive genes could be significantly induced in infected cells.

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