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Comparison of the transcriptomes of tfIIs vs Col-0

ABSTRACT: Testing the consequences of the absence of the transcript elongation factor TFIIS on the transcriptome in Arabidopsis. Keywords: Comparison of mutant and wildtype

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE10663 | GEO | 2009/03/24

SECONDARY ACCESSION(S): PRJNA107631

REPOSITORIES: GEO

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Comparison of the transcriptomes of tfIIs vs Col-0

Project description:Testing the consequences of the absence of the transcript elongation factor TFIIS on the transcriptome in Arabidopsis. Keywords: Comparison of mutant and wildtype Four replicate RNA extractions were performed of each genotype using independent pools of plants. Samples were hybridized in two dye-swaps. tfIIs1#1 (Cy5) vs. Col-0#1 (Cy3) Col-0#2 (Cy5) vs. tfIIs1#2 (Cy3) dye-swap tfIIs1#3 (Cy5) vs. Col-0#3 (Cy3) Col-0#5 (Cy5) vs. tfIIs1#4 (Cy3) dye-swap Samples were hybridized in two dye-swaps. The data were normalized over all 4 hybridizations to obtain one, single log-ratio(Sample/Reference). The raw data files of all four of the hybridizations are attached to the Sample.

2010-04-03 | E-GEOD-10663 | biostudies-arrayexpress

Comparison of the transcriptomes of hmgb1 vs Col-0

Project description:Testing the consequences of the absence of the chromatin-associated HMGB1 protein on the transcriptome in Arabidopsis. Keywords: Comparison of mutant and wildtype samples.

2008-11-24 | GSE10638 | GEO

Comparison of the transcriptomes of hmgb1 vs Col-0

Project description:Testing the consequences of the absence of the chromatin-associated HMGB1 protein on the transcriptome in Arabidopsis. Keywords: Comparison of mutant and wildtype samples. Four replicate RNA extractions were performed of each genotype using independent pools of plants. hmgb1#1 (Cy5) vs. Col-0#1 (Cy3) Col-0#2 (Cy5) vs. hmgb1#2 (Cy3) dye-swap hmgb1#3 (Cy5) vs. Col-0#3 (Cy3) Col-0#5 (Cy5) vs. hmgb1#4 (Cy3) dye-swap Samples were hybridized in two dye-swaps. The data were normalized over all 4 hybridizations to obtain one, single log-ratio(Sample/Reference). The raw data files of all four of the hybridizations are attached to the Sample.

2008-11-23 | E-GEOD-10638 | biostudies-arrayexpress

Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability [ RNA-seq]

Project description:Connections between RNA polymerase II (RNAPII) transcription stress, R-loops, and genome instability have been established however, the underlying mechanisms remain poorly understood. Here we used a mutant version of elongation factor TFIIS (TFIISmut) to specifically induce increased levels of RNAPII pausing, arrest, and/or backtracking in human cells. TFIISmut expression results in slower elongation rates, relative depletion of polymerases from the end of genes, and increased levels of stopped RNAPII. It affects mRNA splicing and termination as well. Remarkably, however, TFIISmut expression also dramatically increases R-loops, which may form at the anterior end of backtracked RNAPII and trigger genome instability, including DNA strand breaks. These results shed new light on the relationship between transcription stress and R-loops, and suggest that different classes of R-loops exist, potentially with distinct consequences for genome instability.

2019-06-13 | GSE132398 | GEO

Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability [TT-seq]

2019-06-13 | GSE132395 | GEO

Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability [ChIP-seq]

2019-06-13 | GSE132391 | GEO

Transcriptomic changes in Arabidopsis thaliana upon the expression of mutated TFIIS

Project description:The expresion of dominant negative version of Arabidopsis TFIIS (TFIISmut) in plants lacking funtional TFIIS leads to severe growth defects. The genome-wide analysis of transcriptomic changes upon TFIISmut expression was performed by RNA-seq to better understand the biological importance of TFIIS. 10DAS seedlings of transgenic lines carring inducible GFP-TFIIS or GFP-TFIISmut transgenes were studied by RNA-seq following plants exposure to 24h b-estradiol or EtOH (mock) induction.

2020-03-25 | GSE129072 | GEO

RNAPII ChIP-Sequencing of A.thaliana tfiis mutant lines under heat stress conditions

Project description:RNAPII ChIP Sequencing in Col-0 and tfiis mutant lines to elucidate the role of TFIIS during RNAPII mediated transcription

2023-12-31 | GSE214303 | GEO

smRNAs in wildtype and RDR6-15 knockout Arabidopsis thaliana Col-0 leaf

Project description:This set consists of small RNAs sequenced from two replicates of wildtype and two replicates of RDR6-15 knockout Arabidopsis thaliana Col-0 leaf samples. RDR6 is required for the production of tasRNAs (trans-acting small RNAS) and so tags associated with the tasRNA loci should be severely down-regulated or absent in the knockout compared to wildtype. The set can thus be used as containing known true positives for testing differential expression detection methods.

2010-11-22 | GSE16959 | GEO

Genome-wide changes in active RNAPII occupancy in the presence of mutated TFIIS (TFIISmut)

Project description:The expresion of dominant negative version of Arabidopsis TFIIS (TFIISmut) in plants lacking funtional TFIIS leads to severe growth defects. The genome-wide analysis of changes in active RNAPII occupancy upon TFIISmut expression was determined using ChIP-seq in order to better understand the biological importance of TFIIS. 10DAS seedlings of transgenic lines carring inducible GFP-TFIIS or GFP-TFIISmut transgenes were studied by ChIP-seq following plants exposure to 24h b-estradiol or EtOH (mock) induction.

2020-03-04 | GSE128316 | GEO

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