Dataset Information


RNA-seq analysis of gene expression in scramble and GPR126 knockdown colon cancer cells (HT-29)

ABSTRACT: Purpose: Identify genes regulated by GPR126 in colon cancer cells by RNA-seq analysis Methods: Use shRNAs to knock down GPR126 in HT-29 cells, total RNAs from scramble group (NC) and GPR126 knockdown group (Sh1) were subjected to RNA-sequencing. Results: Around 700 transcriptomes were up-regulated in GPR126 knockdown HT-29 cells, and 14000 transcriptomes were down-regulated in GPR126 knockdown HT-29 cells.GPR126 mainly regualtes genes from DNA synthesis and cell cycle-related pathways. Conclusions: Our study firstly showed the function of GPR126 regulating colon cancer cell proliferation by targeting genes invovled in DNA synthesis and cell cycle-related pathways. Overall design: RNA interference in colon cancer cell line HT-29, knock down GPR126 expression, and conduct gene expression profiling by RNA-seq. Two groups, one sample from each group.

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

SUBMITTER: Wenjie Yu  

PROVIDER: GSE106696 | GEO | 2017-11-09



Similar Datasets

2012-01-07 | E-GEOD-34926 | ArrayExpress
2012-03-05 | E-GEOD-30817 | ArrayExpress
| PRJNA154569 | ENA
| PRJNA417696 | ENA
2005-08-13 | GSE3099 | GEO
2013-09-17 | E-GEOD-41364 | ArrayExpress
| GSE20147 | GEO
2015-07-08 | E-GEOD-41364 | ExpressionAtlas
2007-09-12 | GSE8967 | GEO
2010-07-07 | E-GEOD-20147 | ArrayExpress