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Wt/myb77-Functional characterization of AtMYB77 transcription factor.

ABSTRACT: au07-03_myb77 - wt/myb77 - Transcriptome analysis of Myb77 knock out mutant line or of Myb77fragment overxpressing transgenic line. - Plants were grown in vitro on 1/2 MS medium, 3% sucrose, 0.8% agar for 21 days under balance day cycle (12 hours daylight at 22°C; 12 hours dark at 20°C) in climate chamber. The whole plants were collected for RNAs extraction. Keywords: gene knock in (transgenic),gene knock out

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE10673 | GEO | 2008/08/13

SECONDARY ACCESSION(S): PRJNA107651

REPOSITORIES: GEO

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wt/myb77-Functional characterization of AtMYB77 transcription factor.

Project description:au07-03_myb77 - wt/myb77 - Transcriptome analysis of Myb77 knock out mutant line or of Myb77fragment overxpressing transgenic line. - Plants were grown in vitro on 1/2 MS medium, 3% sucrose, 0.8% agar for 21 days under balance day cycle (12 hours daylight at 22Â°C; 12 hours dark at 20Â°C) in climate chamber. The whole plants were collected for RNAs extraction. Keywords: gene knock in (transgenic),gene knock out 4 dye-swap - CATMA arrays

2010-05-25 | E-GEOD-10673 | biostudies-arrayexpress

sqe1-Functional analysis and biotechnological application of genes involved in drought and salt stress tolerance

Project description:au07-13_sqe1 - sqe1 - Arabidopsis transcriptome microarray from wild type and mutant plants. - Arabidopsis microarray from Ler ecotype and the sqe-1 mutant. Keywords: gene knock out

2009-01-24 | GSE10349 | GEO

Biodiversity and functional genomics of endogenous small RNAs induced upon biotic and abiotic stresses in plant (gemsup)

Project description:au07-12_geminiselsup - gemsup - Determine the level of expresison in transgenic plants expressing geminviral gene silencing suppressors. - Transgenic lines of Arabidopsis overexpressing three different geminiviral silencing suppressors: C2, C4 and V2 were grown in adition to a control and their total RNA was extracted in order to study their genetic expressions profiles. Keywords: gene knock in (transgenic)

2010-10-12 | GSE24475 | GEO

sqe1-Functional analysis and biotechnological application of genes involved in drought and salt stress tolerance

2009-01-24 | E-GEOD-10349 | biostudies-arrayexpress

Biodiversity and functional genomics of endogenous small RNAs induced upon biotic and abiotic stresses in plant (gemsup)

2010-10-12 | E-GEOD-24475 | biostudies-arrayexpress

Transcription profiling of maize to identify gene expression changes after chilling for 4 hours (alarm phase)

Project description:This experiment evaluates quick (alarm) response to chilling in chilling-sensitive maize plants. Maize inbred line cm109 were grown in optimal conditions until third leaf was fully developed. At this stage plants were divided into three experimental variants: k0 - control plants, frozen at the beginning of daylight, k4 - control plants kept in the same conditions and frozen after 4 hours since beginning of daylight, c4 - plants kept in 14 deg. C for 4 hours since "dawn". At the mentioned moments, leaves were harvested and frozen in liquid nitrogen for RNA isolation.

2009-11-01 | E-MEXP-1875 | biostudies-arrayexpress

Transcriptomic analysis of MAPK KOs mutants under normal growing conditions (bb)

Project description:au07-10_mpk - bb - Gain insight into the function of mpk genes by characterizing the transcriptional profile of the mpk5, mpk10 and mpk11 KO mutants in comparison with that of the Arabidopsis wt plants. - Transcriptomic analysis of the double mutant bak1-4 bkk1-1 to compare its gene expression profile with that of the single mutants atmekk1 and atmpk4 and elucidate their involvement in the brassinolide signalling. Keywords: gene knock out

2009-10-30 | GSE18748 | GEO

Protein turnover rates within mitochondrial complexes in mitochondrial disease patients and in a DNAJC30 knock-out cell line

Project description:We identified DNAJC30 as a key protein in the maintenance of functional NADH:ubiquinone oxidoreductase (complex I). To determine the turnover rates of modules in complex I and other mitochondrial complexes, we pulsed both patient and control fibroblast cell lines and a DNAJC30 knock-out HEK and control HEK cell line over 12 hours with heavy amino acids (PulseSILAC) followed by mass spectrometry.

2021-09-09 | PXD021548 | Pride

Transcriptomic analysis of MAPK KOs mutants under normal growing conditions (bb)

2009-10-30 | E-GEOD-18748 | biostudies-arrayexpress

compare haiku mutants with wildtype-Study of small seed mutants.

Project description:au07-11_haiku - compare haiku mutants with wildtype - What are other genes involved in haiku pathway? - IKU1, IKU2 belong to same pathway which involved in seed development. Using array analysis try to find other components of this pathway. Keywords: gene knock out

2009-07-08 | GSE16956 | GEO

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