Context-dependent effects of Acf1 assessed in high throughput

ABSTRACT: We applied a multiplexed genomic tethering assay (Brueckner, Epigenetics Chromatin, 2016; PMID:27777628) to elucidate the function of ACF1, the large subunit of the ACF nucleosome sliding complex in drosophila Kc167 cells. Targeting a Gal4DBD-ACF1 fusion to several hundred integrated reporters revealed a context-dependent repressive effect which is modulated by gene activity and chromatin state.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE106759 | GEO | 2017/11/10

SECONDARY ACCESSION(S): PRJNA417865

REPOSITORIES: GEO