Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Temporal transcriptomic profiles of transition from hematopoietic multipotent progenitors to B committed cells [RNA-seq]

ABSTRACT: To identify the “time-lapse” TF networks during B lineage commitment, we established multipotent progenitors harboring a tamoxifen-inducible form of Id3, an in vitro system where virtually all cells became B cells within 6 days by simply withdrawing 4-OHT. In this study, transcriptome analysis at multiple time points was performed using the culture system.

ORGANISM(S): Mus musculus

PROVIDER: GSE106793 | GEO | 2018/03/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Temporal epigenomic profiles of transition from hematopoietic multipotent progenitors to B committed cells [ChIP-seq]

Project description:To identify the “time-lapse” TF networks during B lineage commitment, we established multipotent progenitors harboring a tamoxifen-inducible form of Id3, an in vitro system where virtually all cells became B cells within 6 days by simply withdrawing 4-OHT. In this study, epigenomic analysis at multiple time points was performed using the culture system.

2018-03-06 | GSE106794 | GEO

Single cell transcriptome datasets of murine B cell commitment

Project description:To identify the “time-lapse” TF networks during B lineage commitment, we established multipotent progenitors harboring a tamoxifen-inducible form of Id3, an in vitro system where virtually all cells became B cells within 6 days by simply withdrawing 4-OHT. In this study, single cell transcriptomic analysis at pre- and post-commitment was performed using the culture system. In addition, we also performed single cell RNA-seq analysis of B cell precursor populations (LMPP, CLP and pro-B cells) in murine bone marrow.

2018-02-20 | GSE107527 | GEO

Dynamic expression of Id3 defines the stepwise differentiation of tissue-resident regulatory T cells

Project description:Foxp3+ regulatory T (TR) cells are phenotypically and functionally diverse, and broadly distributed in lymphoid and non-lymphoid tissues. However, the pathways guiding the differentiation of tissue-resident TR populations have not been well defined. By regulating E-protein function, Id3 controls the differentiation of CD8+ effector T cells and is essential for TR maintenance and function. We show that dynamic expression of Id3 helps define three distinct mouse TR populations, Id3+CD62LhiCD44lo central (c)TR, Id3+CD62LloCD44hi effector (e)TR and Id3- eTR. Adoptive transfer experiments and transcriptome analyses support a stepwise model of differentiation from Id3+ cTR to Id3+ eTR to Id3- eTR. Furthermore, Id3- eTR have high expression of functional inhibitory markers and a transcriptional signature of tissue-resident TR. Accordingly, Id3- eTR are highly enriched in non-lymphoid organs, but virtually absent from blood and lymph. Thus, we propose that tissue-resident TR develop in a multi-step process associated with Id3 downregulation.

2018-11-16 | GSE122593 | GEO

Expansion of multipotent and lymphoid-committed human progenitors through intracellular dimerization of Mpl.

Project description: Not available

| S-EPMC2288719 | biostudies-literature

Time-lapse SLAM-seq for nuclear RNA

Project description:Time-lapse SLAM-seq for nuclear RNA

| PRJNA646964 | ENA

An epigenetic profile of early T-cell development from multipotent progenitors to committed T-cell descendants.

Project description:Using a stromal cell free system, we described the gene expression and two genome wide epigenetic profiles of a unique population of undifferentiated bone marrow cells selectively driven towards the T cell differentiation pathway

2013-12-25 | GSE47995 | GEO

An epigenetic profile of early T-cell development from multipotent progenitors to committed T-cell descendants.

2013-12-25 | GSE47940 | GEO

Proteomic cornerstones of hematopoietic stem cell differentiation: distinct signatures of multipotent progenitors and myeloid committed cells.

Project description: Not available

| S-EPMC3412962 | biostudies-literature

The Master Regulator Protein BAZ2B Can Reprogram Human Hematopoietic Lineage-Committed Progenitors into a Multipotent State.

Project description: Not available

| S-EPMC8049840 | biostudies-literature

Prospective isolation and characterization of committed and multipotent progenitors from immortalized mouse mammary epithelial cells with morphogenic potential.

Project description: Not available

| S-EPMC3219204 | biostudies-literature