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A lysosome-plasma membrane-sphingolipid axis linking lysosomal storage to cell growth arrest

ABSTRACT: We used human fibroblasts loaded with sucrose as a simple model of lysosomal accumulation, and we verified by high-throughput RNA sequencing which processes were altered by the treatment at two different time points. Moreover, we hypothesized that ceramide production due to the ectopic catabolism of plasma-membrane glycosphingolipids is involved in cell cycle arrest upon sucrose treatment. To test this hypothesis, we treated sucrose-loaded fibroblasts with two specific inhibitors for the β-glucosidases GBA1 and GBA2, and analyzed the cell responses using high-throughput RNA sequence.

ORGANISM(S): Homo sapiens

PROVIDER: GSE106928 | GEO | 2019/11/06

REPOSITORIES: GEO

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A lysosome-plasma membrane-sphingolipid axis linking lysosomal storage to cell growth arrest.

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Project description:A lysosome-plasma membrane-sphingolipid axis linking lysosomal storage to cell growth arrest

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