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The orthologue of the RNA chaperone Hfq is essential for cell motility of the cyanobacterium Synechocystis PCC 6803

ABSTRACT: The gene ssr3341 was previously suggested to encode an orthologue of the RNA chaperone Hfq in Synechocystis sp. strain PCC 6803. When a phototactic strain of this cyanobacterium was insertionally inactivated at ssr3341, the mutants were not transformable, and were rendered non-phototactic compared to the wild type. The loss of motility was complemented by re-introduction of the wild-type gene, correlated with the re-establishment of type IV pili on the cell surface. Microarray analyses revealed a small set of genes with drastically reduced transcript levels in the knock-out mutant over wild-type cells. Among the most strongly affected genes are with slr1667, slr1668, slr2015, slr2016 and slr2018 five genes which belong to two operons that previously were shown to be involved in motility and controlled by the SYCRP1-cAMP receptor protein. This fact constitutes a link between cAMP signalling, motility, and the involvement of RNA-based regulation. This is the first report demonstrating a functional role of Hfq in cyanobacteria. Keywords: comparative expression profiling

ORGANISM(S): Synechocystis sp. PCC 6803

PROVIDER: GSE10708 | GEO | 2008/03/15

SECONDARY ACCESSION(S): PRJNA107587

REPOSITORIES: GEO

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Project description:Physical forces associated with spaceflight and spaceflight analogue culture regulate a wide range of physiological responses by both bacterial and mammalian cells that can impact infection. However, our mechanistic understanding of how these environments regulate host-pathogen interactions in humans is poorly understood. Using a spaceflight analogue low fluid shear culture system, we investigated the effect of Low Shear Modeled Microgravity (LSMMG) culture on the colonization of Salmonella Typhimurium in a 3-D biomimetic model of human colonic epithelium containing macrophages. RNA-seq profiling of stationary phase wild type and hfq mutant bacteria alone indicated that LSMMG culture induced global changes in gene expression in both strains and that the RNA-binding protein Hfq played a significant role in regulating the transcriptional response of the pathogen to LSMMG culture. However, a core set of genes important for adhesion, invasion, and motility were commonly induced in both strains. LSMMG culture enhanced the colonization (adherence, invasion and intracellular survival) of Salmonella in this advanced model of intestinal epithelium using a mechanism that was independent of Hfq. Although S. Typhimurium hfq mutants are normally defective for invasion when grown as conventional shaking cultures, LSMMG conditions unexpectedly enabled high levels of colonization by an isogenic hfq mutant. In response to infection with either the wild type or mutant, host cells upregulated transcripts involved in inflammation, tissue remodeling, and wound healing during intracellular survival. Interestingly, infection by the hfq mutant led to fewer transcriptional differences between LSMMG- and control-infected host cells relative to infection with the wild type strain. This is the first study to investigate the effect of LSMMG culture on the interaction between S. Typhimurium and a 3-D model of human intestinal tissue. These findings advance our understanding of how physical forces can impact the early stages of human enteric salmonellosis.

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The orthologue of the RNA chaperone Hfq is essential for cell motility of the cyanobacterium Synechocystis PCC 6803

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