Dataset Information


Effector CD8 T cells dedifferentiate into long-lived memory cells

ABSTRACT: DNA methyltransferase 3a regulates CD8 T cell memory differentiation Overall design: Transgenic P14 CD8 T cells were harvested from naïve P14 TCR transgenic mice and 2K P14 cells were adoptively transferred intravenously to congenically distinct C57BL/6 mice one day before acute LCMV infection. Effector P14 CD8 T cells were FACS-purified from spleens of acutely infected mice on days 4.5 and 8 post-infection for whole genome bisulfite sequencing analysis (WGBS), and naïve antigen-specific cells obtained from transgenic P14 mice were used as an antigen-specific naïve control for WGBS. Dnmt3a conditional knockout mice were generated by breeding previously characterized floxed Dnmt3a mice with mice that contain a granzyme b driven recombinase transgene. Dnmt3a cKO and WT C57BL/6 mice mice were acutely infected with LCMV Armstrong strain and gp33-specific CD8 T cells were FACS-purified from spleens of acutely infected mice on day 8 post-infection.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: yiping fan  

PROVIDER: GSE107150 | GEO | 2017-12-16



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Memory CD8 T cells that circulate in the blood and are present in lymphoid organs are an essential component of long-lived T cell immunity. These memory CD8 T cells remain poised to rapidly elaborate effector functions upon re-exposure to pathogens, but also have many properties in common with naive cells, including pluripotency and the ability to migrate to the lymph nodes and spleen. Thus, memory cells embody features of both naive and effector cells, fuelling a long-standing debate centred on  ...[more]

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